Detailed information of TA system TA196166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 588372..588967
• Replicon: chromosome
• Accession: NZ_CP071311
• Organism: Rhizobium sp. X9

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JZY08_RS02815
• Protein ID: WP_082184620.1
• Coordinates: 588668..588967 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7L5BFT0
• Locus tag: JZY08_RS02810
• Protein ID: WP_035242036.1
• Coordinates: 588372..588665 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZY08_RS02785	583416..584414	+	999	WP_216330935.1	asparaginase	-
JZY08_RS02790	584555..585175	+	621	WP_003494332.1	glutathione S-transferase family protein	-
JZY08_RS02795	585396..586055	-	660	WP_216330936.1	glutathione S-transferase family protein	-
JZY08_RS02800	586139..586654	-	516	WP_003494334.1	MarR family transcriptional regulator	-
JZY08_RS02805	586795..588327	+	1533	WP_216330937.1	MDR family MFS transporter	-
JZY08_RS02810	588372..588665	-	294	WP_035242036.1	putative addiction module antidote protein	Antitoxin
JZY08_RS02815	588668..588967	-	300	WP_082184620.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JZY08_RS02820	589205..590221	+	1017	WP_216330938.1	ligase-associated DNA damage response exonuclease	-
JZY08_RS02825	590223..591848	+	1626	WP_216330939.1	cisplatin damage response ATP-dependent DNA ligase	-
JZY08_RS02830	592102..593070	+	969	WP_216330940.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-96)

Antitoxin

• Uncharacterized protein HI1420 (4-95)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11205.98 Da
• Isoelectric Point: 10.4207

>T196166 WP_082184620.1 NZ_CP071311:c588967-588668 [Rhizobium sp. X9]
MNSFERSAEFDEWLKALKDRTGKVRILHRLTAAKHGNFGDCEPVGGGVSEMRIHYGPGYRVYFTRIGKKVYFLLIGGDKS
SQKRDIKLAIDMAQTIGKK

Nucleotide

• Length: 300 bp

>T196166 NZ_CP095022:c753880-753572 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10659.18 Da
• Isoelectric Point: 4.8307

>AT196166 WP_035242036.1 NZ_CP071311:c588665-588372 [Rhizobium sp. X9]
MTEFSKFDVSDYLDSDETMIEYLAVAMEDENPDVFIAALGAVARARGMSHIADVSGLGRESLYKALKPGSKLRYETVQKV
MHALGFRLAVEKSLNAA

Nucleotide

• Length: 294 bp

>AT196166 NZ_CP095022:c754112-753867 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7L5BFT0