Detailed information of TA system TA196159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1127248..1128356
• Replicon: chromosome
• Accession: NZ_CP071301
• Organism: Lactococcus sp. LG606

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A2H4HI44
• Locus tag: J0J35_RS05495
• Protein ID: WP_029170889.1
• Coordinates: 1127248..1128117 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: J0J35_RS05500
• Protein ID: WP_000205227.1
• Coordinates: 1128132..1128356 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0J35_RS05455	1122623..1122796	-	174	WP_171902975.1	rRNA adenine methyltransferase	-
J0J35_RS05460	1122741..1123478	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
J0J35_RS05465	1123603..1123686	-	84	WP_032501549.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
J0J35_RS05470	1123735..1123818	-	84	Protein_1084	peptide-binding protein	-
J0J35_RS05475	1124228..1125022	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
J0J35_RS05480	1125115..1125595	-	481	Protein_1086	GNAT family N-acetyltransferase	-
J0J35_RS05485	1125592..1126500	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
J0J35_RS05490	1126533..1127267	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
J0J35_RS05495	1127248..1128117	-	870	WP_029170889.1	nucleotidyltransferase domain-containing protein	Toxin
J0J35_RS05500	1128132..1128356	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
J0J35_RS05505	1128499..1130043	-	1545	WP_206918171.1	recombinase family protein	-
J0J35_RS05510	1130063..1130479	-	417	WP_000323438.1	recombinase	-
J0J35_RS05515	1130480..1130776	-	297	WP_002360685.1	hypothetical protein	-
J0J35_RS05520	1131289..1131966	-	678	Protein_1094	DNA topoisomerase	-
J0J35_RS05525	1131982..1132584	-	603	WP_001062586.1	recombinase family protein	-
J0J35_RS05530	1132598..1132768	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32820.25 Da
• Isoelectric Point: 4.9564

>T196159 WP_029170889.1 NZ_CP071301:c1128117-1127248 [Lactococcus sp. LG606]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T196159 NZ_CP095022:c693585-693193 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT196159 WP_000205227.1 NZ_CP071301:c1128356-1128132 [Lactococcus sp. LG606]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT196159 NZ_CP095022:c693839-693582 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2H4HI44