Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 16886..17657 Replicon plasmid pLG1074-optrA
Accession NZ_CP071294
Organism Lactococcus sp. LG1074

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag J0J36_RS10110 Protein ID WP_206886832.1
Coordinates 17160..17657 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag J0J36_RS10105 Protein ID WP_000301765.1
Coordinates 16886..17158 (+) Length 91 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0J36_RS10080 13207..13443 - 237 WP_001159903.1 hypothetical protein -
J0J36_RS10085 13685..13831 + 147 Protein_15 topoisomerase I -
J0J36_RS10090 13880..15556 + 1677 Protein_16 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
J0J36_RS10095 15659..16555 + 897 WP_002387620.1 ParA family protein -
J0J36_RS10100 16660..16869 + 210 WP_000527317.1 peptide-binding protein -
J0J36_RS10105 16886..17158 + 273 WP_000301765.1 antitoxin Antitoxin
J0J36_RS10110 17160..17657 + 498 WP_206886832.1 zeta toxin family protein Toxin
J0J36_RS10115 17711..18391 + 681 WP_206886833.1 IS6 family transposase -
J0J36_RS10120 18579..19040 - 462 Protein_22 aminoglycoside phosphotransferase family protein -
J0J36_RS10125 19600..21567 - 1968 WP_078122475.1 ABC-F type ribosomal protection protein OptrA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrG / optrA / fexA / erm(B) / tet(L) - 1..85819 85819


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-159)

Antitoxin

(2-90)


Sequences


Toxin        


Download         Length: 166 a.a.        Molecular weight: 18409.88 Da        Isoelectric Point: 5.7916

>T196158 WP_206886832.1 NZ_CP071294:17160-17657 [Lactococcus sp. LG1074]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYG
SVAKF

Download         Length: 498 bp

>T196158 NZ_CP095022:676275-676682 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT196158 WP_000301765.1 NZ_CP071294:16886-17158 [Lactococcus sp. LG1074]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT196158 NZ_CP095022:676063-676278 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References