Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58669..59405 Replicon plasmid pSA-KpST14-3
Accession NZ_CP071282
Organism Klebsiella pneumoniae subsp. pneumoniae strain SA-KpST14

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JZ794_RS28020 Protein ID WP_003026803.1
Coordinates 58923..59405 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JZ794_RS28015 Protein ID WP_003026799.1
Coordinates 58669..58935 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZ794_RS27970 54731..55093 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
JZ794_RS27975 55143..55493 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JZ794_RS27980 55851..56120 + 270 WP_004152102.1 hypothetical protein -
JZ794_RS27985 56108..56683 + 576 WP_004152103.1 hypothetical protein -
JZ794_RS27990 56714..57208 + 495 WP_004152104.1 DNA-binding protein -
JZ794_RS27995 57252..57620 + 369 WP_004152105.1 hypothetical protein -
JZ794_RS28000 57654..57857 + 204 WP_004152106.1 hemolysin expression modulator Hha -
JZ794_RS28005 57906..58163 + 258 WP_004152107.1 hypothetical protein -
JZ794_RS28010 58239..58493 + 255 WP_004152108.1 hypothetical protein -
JZ794_RS28015 58669..58935 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JZ794_RS28020 58923..59405 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JZ794_RS28025 59606..60742 + 1137 Protein_64 ISNCY-like element ISKpn21 family transposase -
JZ794_RS28030 60805..61509 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
JZ794_RS28035 61745..62176 + 432 WP_004152641.1 conjugation system SOS inhibitor PsiB -
JZ794_RS28040 62173..62901 + 729 WP_004152640.1 plasmid SOS inhibition protein A -
JZ794_RS28045 62898..63224 + 327 WP_004152639.1 hypothetical protein -
JZ794_RS28050 63280..63654 + 375 WP_004152638.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..166565 166565


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T196157 WP_003026803.1 NZ_CP071282:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T196157 NZ_CP095022:c639000-638587 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT196157 WP_003026799.1 NZ_CP071282:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT196157 NZ_CP095022:c639263-638997 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References