Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4955742..4956367 Replicon chromosome
Accession NZ_CP071279
Organism Klebsiella pneumoniae subsp. pneumoniae strain SA-KpST14

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JZ794_RS23960 Protein ID WP_002882817.1
Coordinates 4955742..4956125 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JZ794_RS23965 Protein ID WP_004150355.1
Coordinates 4956125..4956367 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZ794_RS23945 4953108..4954010 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JZ794_RS23950 4954007..4954642 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JZ794_RS23955 4954639..4955568 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JZ794_RS23960 4955742..4956125 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JZ794_RS23965 4956125..4956367 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JZ794_RS23970 4956572..4957489 + 918 WP_004181612.1 alpha/beta hydrolase -
JZ794_RS23975 4957503..4958444 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JZ794_RS23980 4958489..4958926 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JZ794_RS23985 4958923..4959783 - 861 WP_004146232.1 virulence factor BrkB family protein -
JZ794_RS23990 4959777..4960376 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T196153 WP_002882817.1 NZ_CP071279:c4956125-4955742 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T196153 NZ_CP095022:361890-362192 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT196153 WP_004150355.1 NZ_CP071279:c4956367-4956125 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT196153 NZ_CP095022:361666-361893 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGAGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References