Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4732128..4732744 Replicon chromosome
Accession NZ_CP071265
Organism Citrobacter freundii strain MEI002

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag J0G25_RS22575 Protein ID WP_003028682.1
Coordinates 4732128..4732502 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag J0G25_RS22580 Protein ID WP_043018956.1
Coordinates 4732502..4732744 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G25_RS22560 (4729631) 4729631..4730533 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
J0G25_RS22565 (4730530) 4730530..4731165 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
J0G25_RS22570 (4731162) 4731162..4732091 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
J0G25_RS22575 (4732128) 4732128..4732502 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
J0G25_RS22580 (4732502) 4732502..4732744 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
J0G25_RS22585 (4732950) 4732950..4733858 + 909 WP_044701816.1 alpha/beta hydrolase -
J0G25_RS22590 (4734009) 4734009..4734950 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
J0G25_RS22595 (4734995) 4734995..4735432 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
J0G25_RS22600 (4735429) 4735429..4736301 - 873 WP_003028669.1 virulence factor BrkB family protein -
J0G25_RS22605 (4736295) 4736295..4736894 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T196134 WP_003028682.1 NZ_CP071265:c4732502-4732128 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T196134 NZ_CP095021:c2845204-2844785 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT196134 WP_043018956.1 NZ_CP071265:c4732744-4732502 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT196134 NZ_CP095021:c2845425-2845201 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References