Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 4580194..4580860 Replicon chromosome
Accession NZ_CP071265
Organism Citrobacter freundii strain MEI002

Toxin (Protein)


Gene name tad Uniprot ID A0A0D7LIM5
Locus tag J0G25_RS21890 Protein ID WP_044702260.1
Coordinates 4580501..4580860 (-) Length 120 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID A0A0D7LIR0
Locus tag J0G25_RS21885 Protein ID WP_003844682.1
Coordinates 4580194..4580511 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G25_RS21855 (4575861) 4575861..4576685 + 825 WP_003031641.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
J0G25_RS21860 (4576754) 4576754..4577977 + 1224 WP_044702265.1 L-sorbose 1-phosphate reductase -
J0G25_RS21865 (4577979) 4577979..4578791 + 813 WP_044702264.1 shikimate 5-dehydrogenase -
J0G25_RS21870 (4578843) 4578843..4579199 - 357 WP_032948294.1 hypothetical protein -
J0G25_RS21875 (4579341) 4579341..4579502 + 162 WP_003841416.1 phage protein -
J0G25_RS21880 (4579906) 4579906..4580184 + 279 WP_044702262.1 putative addiction module antidote protein -
J0G25_RS21885 (4580194) 4580194..4580511 - 318 WP_003844682.1 helix-turn-helix transcriptional regulator Antitoxin
J0G25_RS21890 (4580501) 4580501..4580860 - 360 WP_044702260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J0G25_RS21895 (4581074) 4581074..4581763 + 690 WP_003841421.1 dipeptidase PepE -
J0G25_RS21900 (4581829) 4581829..4583460 - 1632 WP_032938230.1 Na/Pi cotransporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-90)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13437.49 Da        Isoelectric Point: 10.2555

>T196133 WP_044702260.1 NZ_CP071265:c4580860-4580501 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Download         Length: 360 bp

>T196133 NZ_CP095021:2798831-2799256 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGCGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11466.35 Da        Isoelectric Point: 9.0005

>AT196133 WP_003844682.1 NZ_CP071265:c4580511-4580194 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Download         Length: 318 bp

>AT196133 NZ_CP095021:2798604-2798825 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7LIM5

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LIR0

References