Detailed information of TA system TA196132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4324056..4324572
• Replicon: chromosome
• Accession: NZ_CP071265
• Organism: Citrobacter freundii strain MEI002

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J0G25_RS20670
• Protein ID: WP_044699148.1
• Coordinates: 4324056..4324340 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0J1MV10
• Locus tag: J0G25_RS20675
• Protein ID: WP_003839576.1
• Coordinates: 4324330..4324572 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G25_RS20655 (4320381)	4320381..4320965	+	585	WP_003839584.1	fructose PTS transporter subunit IIA	-
J0G25_RS20660 (4321343)	4321343..4323481	+	2139	WP_003025782.1	anaerobic ribonucleoside-triphosphate reductase	-
J0G25_RS20665 (4323588)	4323588..4324052	+	465	WP_032937736.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
J0G25_RS20670 (4324056)	4324056..4324340	-	285	WP_044699148.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J0G25_RS20675 (4324330)	4324330..4324572	-	243	WP_003839576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
J0G25_RS20680 (4324650)	4324650..4326563	-	1914	WP_003839574.1	BglG family transcription antiterminator	-
J0G25_RS20685 (4326585)	4326585..4327325	-	741	WP_044699149.1	KDGP aldolase family protein	-
J0G25_RS20690 (4327322)	4327322..4328440	-	1119	WP_044699152.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
J0G25_RS20695 (4328424)	4328424..4329557	-	1134	WP_016149471.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10865.69 Da
• Isoelectric Point: 10.1988

>T196132 WP_044699148.1 NZ_CP071265:c4324340-4324056 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVTVFVVAVGK
RQHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T196132 NZ_CP095021:c2402404-2402087 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.34 Da
• Isoelectric Point: 5.0651

>AT196132 WP_003839576.1 NZ_CP071265:c4324572-4324330 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT196132 NZ_CP095021:c2402616-2402401 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MV10