Detailed information of TA system TA196131

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yafW/CbtA-CbeA
• Location: 4257343..4258022
• Replicon: chromosome
• Accession: NZ_CP071265
• Organism: Citrobacter freundii strain MEI002

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: J0G25_RS20360
• Protein ID: WP_063844629.1
• Coordinates: 4257343..4257684 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: J0G25_RS20365
• Protein ID: WP_063844628.1
• Coordinates: 4257705..4258022 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G25_RS20340 (4253832)	4253832..4254194	+	363	WP_044699097.1	endoribonuclease SymE	-
J0G25_RS20345 (4254248)	4254248..4254883	-	636	WP_044699099.1	HNH endonuclease	-
J0G25_RS20350 (4255024)	4255024..4255896	+	873	WP_080942000.1	HNH endonuclease	-
J0G25_RS20355 (4256077)	4256077..4257084	-	1008	WP_063844630.1	restriction endonuclease	-
J0G25_RS20360 (4257343)	4257343..4257684	-	342	WP_063844629.1	TA system toxin CbtA family protein	Toxin
J0G25_RS20365 (4257705)	4257705..4258022	-	318	WP_063844628.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J0G25_RS20370 (4258041)	4258041..4258262	-	222	WP_007893437.1	DUF987 domain-containing protein	-
J0G25_RS20375 (4258271)	4258271..4258747	-	477	WP_063844627.1	RadC family protein	-
J0G25_RS20380 (4258763)	4258763..4259221	-	459	WP_063844626.1	antirestriction protein	-
J0G25_RS20385 (4259323)	4259323..4259562	-	240	WP_063844625.1	DUF905 domain-containing protein	-
J0G25_RS20390 (4259639)	4259639..4260106	-	468	WP_063844624.1	hypothetical protein	-
J0G25_RS20395 (4260130)	4260130..4260573	-	444	WP_088169063.1	hypothetical protein	-
J0G25_RS20400 (4260563)	4260563..4260808	-	246	WP_032294624.1	protein YpjK	-
J0G25_RS20405 (4260849)	4260849..4261550	-	702	WP_063844622.1	WYL domain-containing protein	-
J0G25_RS20410 (4261767)	4261767..4262588	-	822	WP_063844621.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4257343..4277236	19893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12761.71 Da
• Isoelectric Point: 8.0324

>T196131 WP_063844629.1 NZ_CP071265:c4257684-4257343 [Citrobacter freundii]
MKTLPATNPQVATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T196131 NZ_CP095021:c2362884-2362450 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCGCAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11987.59 Da
• Isoelectric Point: 7.0268

>AT196131 WP_063844628.1 NZ_CP071265:c4258022-4257705 [Citrobacter freundii]
MSNPTRGLQREITPRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAYCVTLYH
NDLTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 318 bp

>AT196131 NZ_CP095021:c2363145-2362891 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG