196130

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3875332..3876011 Replicon chromosome
Accession NZ_CP071265
Organism Citrobacter freundii strain MEI002

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag J0G25_RS18630 Protein ID WP_003031349.1
Coordinates 3875670..3876011 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag J0G25_RS18625 Protein ID WP_003031347.1
Coordinates 3875332..3875649 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G25_RS18600 (3871425) 3871425..3873422 - 1998 WP_016245729.1 choline BCCT transporter BetT -
J0G25_RS18605 (3873970) 3873970..3874117 + 148 Protein_3650 hypothetical protein -
J0G25_RS18610 (3874131) 3874131..3874592 + 462 WP_003031344.1 antirestriction protein -
J0G25_RS18615 (3874608) 3874608..3875084 + 477 WP_003031345.1 RadC family protein -
J0G25_RS18620 (3875093) 3875093..3875314 + 222 WP_003031346.1 DUF987 domain-containing protein -
J0G25_RS18625 (3875332) 3875332..3875649 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
J0G25_RS18630 (3875670) 3875670..3876011 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
J0G25_RS18640 (3876641) 3876641..3876949 - 309 WP_230132591.1 isoprenylcysteine carboxylmethyltransferase family protein -
J0G25_RS18645 (3877297) 3877297..3878745 - 1449 WP_044702495.1 EAL domain-containing protein -
J0G25_RS18650 (3879087) 3879087..3879560 - 474 WP_044702484.1 hypothetical protein -
J0G25_RS18655 (3879535) 3879535..3880266 - 732 WP_044702485.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3860959..3887907 26948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T196130 WP_003031349.1 NZ_CP071265:3875670-3876011 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T196130 NZ_CP095021:2319343-2319753 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT196130 WP_003031347.1 NZ_CP071265:3875332-3875649 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT196130 NZ_CP095021:2319117-2319350 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References