Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3639678..3640298 Replicon chromosome
Accession NZ_CP071265
Organism Citrobacter freundii strain MEI002

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag J0G25_RS17530 Protein ID WP_002892050.1
Coordinates 3640080..3640298 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag J0G25_RS17525 Protein ID WP_003021733.1
Coordinates 3639678..3640052 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G25_RS17515 (3634825) 3634825..3636018 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J0G25_RS17520 (3636041) 3636041..3639190 + 3150 WP_003021736.1 efflux RND transporter permease AcrB -
J0G25_RS17525 (3639678) 3639678..3640052 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
J0G25_RS17530 (3640080) 3640080..3640298 + 219 WP_002892050.1 HHA domain-containing protein Toxin
J0G25_RS17535 (3640605) 3640605..3641156 + 552 WP_044699372.1 maltose O-acetyltransferase -
J0G25_RS17540 (3641273) 3641273..3641743 + 471 WP_003021724.1 YlaC family protein -
J0G25_RS17545 (3641822) 3641822..3641962 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
J0G25_RS17550 (3641964) 3641964..3642224 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
J0G25_RS17555 (3642413) 3642413..3643966 + 1554 WP_044699371.1 EAL domain-containing protein -
J0G25_RS17560 (3644018) 3644018..3644371 - 354 WP_003831033.1 DUF1428 family protein -
J0G25_RS17565 (3644436) 3644436..3645065 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196129 WP_002892050.1 NZ_CP071265:3640080-3640298 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T196129 NZ_CP095021:2250202-2250600 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT196129 WP_003021733.1 NZ_CP071265:3639678-3640052 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT196129 NZ_CP095021:2249974-2250201 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References