Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 850565..851219 Replicon chromosome
Accession NZ_CP071265
Organism Citrobacter freundii strain MEI002

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag J0G25_RS04150 Protein ID WP_003026936.1
Coordinates 850812..851219 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag J0G25_RS04145 Protein ID WP_003026938.1
Coordinates 850565..850831 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G25_RS04120 (845766) 845766..847199 - 1434 WP_044700805.1 6-phospho-beta-glucosidase BglA -
J0G25_RS04125 (847320) 847320..848048 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
J0G25_RS04130 (848101) 848101..848412 + 312 WP_044700802.1 N(4)-acetylcytidine aminohydrolase -
J0G25_RS04135 (848575) 848575..849234 + 660 WP_003838267.1 hemolysin III family protein -
J0G25_RS04140 (849328) 849328..850308 - 981 WP_003026944.1 tRNA-modifying protein YgfZ -
J0G25_RS04145 (850565) 850565..850831 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
J0G25_RS04150 (850812) 850812..851219 + 408 WP_003026936.1 protein YgfX Toxin
J0G25_RS04155 (851264) 851264..851785 - 522 WP_003026933.1 flavodoxin FldB -
J0G25_RS04160 (851899) 851899..852795 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
J0G25_RS04165 (852819) 852819..853532 + 714 WP_003026923.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J0G25_RS04170 (853538) 853538..855271 + 1734 WP_044700800.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T196119 WP_003026936.1 NZ_CP071265:850812-851219 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T196119 NZ_CP095021:1691223-1691540 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT196119 WP_003026938.1 NZ_CP071265:850565-850831 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT196119 NZ_CP095021:1690924-1691226 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References