Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4259872..4260490 Replicon chromosome
Accession NZ_CP071263
Organism Escherichia coli strain MEI003

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J0G23_RS20470 Protein ID WP_001291435.1
Coordinates 4259872..4260090 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J0G23_RS20475 Protein ID WP_000344800.1
Coordinates 4260116..4260490 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G23_RS20435 (4255161) 4255161..4255733 + 573 WP_000779831.1 YbaY family lipoprotein -
J0G23_RS20440 (4255764) 4255764..4256075 - 312 WP_000409911.1 MGMT family protein -
J0G23_RS20450 (4256454) 4256454..4256807 + 354 WP_000878140.1 DUF1428 domain-containing protein -
J0G23_RS20455 (4256849) 4256849..4258399 - 1551 WP_024229235.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J0G23_RS20460 (4258563) 4258563..4259033 - 471 WP_000136192.1 YlaC family protein -
J0G23_RS20465 (4259149) 4259149..4259700 - 552 WP_024229234.1 maltose O-acetyltransferase -
J0G23_RS20470 (4259872) 4259872..4260090 - 219 WP_001291435.1 HHA domain-containing protein Toxin
J0G23_RS20475 (4260116) 4260116..4260490 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J0G23_RS20480 (4261036) 4261036..4264185 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
J0G23_RS20485 (4264208) 4264208..4265401 - 1194 WP_024230275.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196111 WP_001291435.1 NZ_CP071263:c4260090-4259872 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T196111 NZ_CP095021:840123-840551 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT196111 WP_000344800.1 NZ_CP071263:c4260490-4260116 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT196111 NZ_CP095021:839842-840099 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References