Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1950572..1951226 Replicon chromosome
Accession NZ_CP071263
Organism Escherichia coli strain MEI003

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag J0G23_RS09520 Protein ID WP_000244769.1
Coordinates 1950572..1950979 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J0G23_RS09525 Protein ID WP_000354046.1
Coordinates 1950960..1951226 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G23_RS09500 (1946529) 1946529..1948262 - 1734 WP_024229618.1 single-stranded-DNA-specific exonuclease RecJ -
J0G23_RS09505 (1948268) 1948268..1948978 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J0G23_RS09510 (1949003) 1949003..1949899 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
J0G23_RS09515 (1950011) 1950011..1950532 + 522 WP_152079814.1 flavodoxin FldB -
J0G23_RS09520 (1950572) 1950572..1950979 - 408 WP_000244769.1 protein YgfX Toxin
J0G23_RS09525 (1950960) 1950960..1951226 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J0G23_RS09530 (1951475) 1951475..1952455 + 981 WP_024229619.1 tRNA-modifying protein YgfZ -
J0G23_RS09535 (1952532) 1952532..1953191 - 660 WP_000250274.1 hemolysin III family protein -
J0G23_RS09540 (1953355) 1953355..1953666 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
J0G23_RS09545 (1953711) 1953711..1955144 + 1434 WP_024229620.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T196096 WP_000244769.1 NZ_CP071263:c1950979-1950572 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T196096 NZ_CP095021:361895-362197 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT196096 WP_000354046.1 NZ_CP071263:c1951226-1950960 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT196096 NZ_CP095021:362245-362466 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References