Detailed information of TA system TA196081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 366349..367937
• Replicon: chromosome
• Accession: NZ_CP071263
• Organism: Escherichia coli strain MEI003

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A828NFN0
• Locus tag: J0G23_RS01835
• Protein ID: WP_024229884.1
• Coordinates: 366349..367671 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J0G23_RS01840
• Protein ID: WP_001296726.1
• Coordinates: 367671..367937 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G23_RS01800 (361383)	361383..361955	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
J0G23_RS01805 (362106)	362106..362669	-	564	WP_077828246.1	amidohydrolase family protein	-
J0G23_RS01810 (362629)	362629..362784	-	156	WP_001322415.1	hypothetical protein	-
J0G23_RS01815 (362887)	362887..363834	-	948	WP_000854796.1	aldo/keto reductase	-
J0G23_RS01820 (363908)	363908..364597	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
J0G23_RS01825 (364606)	364606..364914	-	309	WP_001221046.1	hypothetical protein	-
J0G23_RS01830 (365054)	365054..365953	+	900	WP_001057776.1	LysR family transcriptional regulator	-
J0G23_RS01835 (366349)	366349..367671	-	1323	WP_024229884.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
J0G23_RS01840 (367671)	367671..367937	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49414.07 Da
• Isoelectric Point: 8.5830

>T196081 WP_024229884.1 NZ_CP071263:c367671-366349 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTTIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T196081 NZ_CP095020:c2970177-2969854 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT196081 WP_001296726.1 NZ_CP071263:c367937-367671 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT196081 NZ_CP095020:c2970502-2970257 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Structures

Toxin

• AlphaFold DB: A0A828NFN0