Detailed information of TA system TA196070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3968937..3969556
• Replicon: chromosome
• Accession: NZ_CP071261
• Organism: Klebsiella pneumoniae strain MEI004

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: J0G27_RS19335
• Protein ID: WP_002892050.1
• Coordinates: 3969338..3969556 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: J0G27_RS19330
• Protein ID: WP_250354731.1
• Coordinates: 3968937..3969311 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G27_RS19320 (3964089)	3964089..3965282	+	1194	WP_002892072.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
J0G27_RS19325 (3965305)	3965305..3968451	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
J0G27_RS19330 (3968937)	3968937..3969311	+	375	WP_250354731.1	Hha toxicity modulator TomB	Antitoxin
J0G27_RS19335 (3969338)	3969338..3969556	+	219	WP_002892050.1	HHA domain-containing protein	Toxin
J0G27_RS19340 (3969715)	3969715..3970281	+	567	WP_002892030.1	maltose O-acetyltransferase	-
J0G27_RS19345 (3970253)	3970253..3970393	-	141	WP_004147370.1	hypothetical protein	-
J0G27_RS19350 (3970414)	3970414..3970884	+	471	WP_002892026.1	YlaC family protein	-
J0G27_RS19355 (3970859)	3970859..3972310	-	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
J0G27_RS19360 (3972411)	3972411..3973109	+	699	WP_004178762.1	GNAT family protein	-
J0G27_RS19365 (3973106)	3973106..3973246	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
J0G27_RS19370 (3973246)	3973246..3973509	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T196070 WP_002892050.1 NZ_CP071261:3969338-3969556 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T196070 NZ_CP095020:c2227036-2226692 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14368.06 Da
• Isoelectric Point: 4.8989

>AT196070 WP_250354731.1 NZ_CP071261:3968937-3969311 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLLAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT196070 NZ_CP095020:c2227278-2227030 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2