Detailed information of TA system TA196068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2624523..2626111
• Replicon: chromosome
• Accession: NZ_CP071261
• Organism: Klebsiella pneumoniae strain MEI004

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J0G27_RS13005
• Protein ID: WP_250354974.1
• Coordinates: 2624523..2625845 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: J0G27_RS13010
• Protein ID: WP_002904790.1
• Coordinates: 2625845..2626111 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G27_RS12990 (2619587)	2619587..2622088	+	2502	WP_250354972.1	fimbria/pilus outer membrane usher protein	-
J0G27_RS12995 (2622076)	2622076..2623080	+	1005	WP_250354973.1	fimbrial protein	-
J0G27_RS13000 (2623214)	2623214..2624506	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
J0G27_RS13005 (2624523)	2624523..2625845	-	1323	WP_250354974.1	type II toxin-antitoxin system HipA family toxin	Toxin
J0G27_RS13010 (2625845)	2625845..2626111	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J0G27_RS13015 (2626376)	2626376..2626702	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
J0G27_RS13020 (2626699)	2626699..2627199	-	501	WP_023282573.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
J0G27_RS13025 (2627177)	2627177..2628556	-	1380	WP_086519587.1	nucleobase:cation symporter-2 family protein	-
J0G27_RS13030 (2628902)	2628902..2630056	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
J0G27_RS13035 (2630037)	2630037..2630960	-	924	WP_004184020.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49122.44 Da
• Isoelectric Point: 7.5618

>T196068 WP_250354974.1 NZ_CP071261:c2625845-2624523 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLASAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEIHAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T196068 NZ_CP095020:c2218219-2217800 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT196068 WP_002904790.1 NZ_CP071261:c2626111-2625845 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT196068 NZ_CP095020:c2218488-2218228 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8