Detailed information of TA system TA196054

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4670316..4671148
• Replicon: chromosome
• Accession: NZ_CP071259
• Organism: Escherichia coli strain MEI005

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: J0G24_RS22625
• Protein ID: WP_000854753.1
• Coordinates: 4670774..4671148 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: J0G24_RS22620
• Protein ID: WP_001278232.1
• Coordinates: 4670316..4670684 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G24_RS22585 (4666159)	4666159..4666839	+	681	WP_001282919.1	WYL domain-containing protein	-
J0G24_RS22590 (4666987)	4666987..4667664	+	678	WP_001097301.1	hypothetical protein	-
J0G24_RS22595 (4667670)	4667670..4667903	+	234	WP_001278287.1	DUF905 family protein	-
J0G24_RS22600 (4668002)	4668002..4668820	+	819	WP_001234631.1	DUF932 domain-containing protein	-
J0G24_RS22605 (4668902)	4668902..4669381	+	480	WP_000860065.1	antirestriction protein	-
J0G24_RS22610 (4669393)	4669393..4669869	+	477	WP_001186710.1	RadC family protein	-
J0G24_RS22615 (4669932)	4669932..4670153	+	222	WP_000692303.1	DUF987 domain-containing protein	-
J0G24_RS22620 (4670316)	4670316..4670684	+	369	WP_001278232.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
J0G24_RS22625 (4670774)	4670774..4671148	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
J0G24_RS22630 (4671145)	4671145..4671633	+	489	WP_000777541.1	DUF5983 family protein	-
J0G24_RS22635 (4671645)	4671645..4671842	+	198	WP_000839281.1	DUF957 domain-containing protein	-
J0G24_RS22640 (4671939)	4671939..4672508	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
J0G24_RS22645 (4673257)	4673257..4674795	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	iroB	4637024..4682608	45584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T196054 WP_000854753.1 NZ_CP071259:4670774-4671148 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T196054 NZ_CP095020:1013608-1014042 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT196054 WP_001278232.1 NZ_CP071259:4670316-4670684 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT196054 NZ_CP095020:1013423-1013602 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72