Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3761914..3762532 Replicon chromosome
Accession NZ_CP071259
Organism Escherichia coli strain MEI005

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J0G24_RS18390 Protein ID WP_001291435.1
Coordinates 3762314..3762532 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J0G24_RS18385 Protein ID WP_000344800.1
Coordinates 3761914..3762288 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G24_RS18375 (3757003) 3757003..3758196 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J0G24_RS18380 (3758219) 3758219..3761368 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J0G24_RS18385 (3761914) 3761914..3762288 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J0G24_RS18390 (3762314) 3762314..3762532 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J0G24_RS18395 (3762704) 3762704..3763255 + 552 WP_000102568.1 maltose O-acetyltransferase -
J0G24_RS18400 (3763371) 3763371..3763841 + 471 WP_000136192.1 YlaC family protein -
J0G24_RS18405 (3764005) 3764005..3765555 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J0G24_RS18410 (3765597) 3765597..3765950 - 354 WP_000878140.1 DUF1428 domain-containing protein -
J0G24_RS18420 (3766329) 3766329..3766640 + 312 WP_000409911.1 MGMT family protein -
J0G24_RS18425 (3766671) 3766671..3767243 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196051 WP_001291435.1 NZ_CP071259:3762314-3762532 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T196051 NZ_CP095020:c754816-754379 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT196051 WP_000344800.1 NZ_CP071259:3761914-3762288 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT196051 NZ_CP095020:c754269-754024 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References