Detailed information of TA system TA196050

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2998763..2999561
• Replicon: chromosome
• Accession: NZ_CP071259
• Organism: Escherichia coli strain MEI005

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8T6QC23
• Locus tag: J0G24_RS14645
• Protein ID: WP_042022646.1
• Coordinates: 2998763..2999140 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: J0G24_RS14650
• Protein ID: WP_001285482.1
• Coordinates: 2999187..2999561 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G24_RS14610 (2994350)	2994350..2994904	-	555	WP_001001902.1	molecular chaperone YcdY	-
J0G24_RS14615 (2994928)	2994928..2995665	-	738	WP_000283664.1	zinc-binding phosphatase	-
J0G24_RS14620 (2995720)	2995720..2996658	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
J0G24_RS14630 (2997129)	2997129..2997972	-	844	Protein_2875	DUF4942 domain-containing protein	-
J0G24_RS14635 (2998069)	2998069..2998266	-	198	WP_042022639.1	DUF957 domain-containing protein	-
J0G24_RS14640 (2998263)	2998263..2998766	-	504	WP_061335745.1	DUF5983 family protein	-
J0G24_RS14645 (2998763)	2998763..2999140	-	378	WP_042022646.1	TA system toxin CbtA family protein	Toxin
J0G24_RS14650 (2999187)	2999187..2999561	-	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J0G24_RS14655 (2999611)	2999611..3000255	-	645	WP_000086759.1	hypothetical protein	-
J0G24_RS14660 (3000274)	3000274..3000495	-	222	WP_000692345.1	DUF987 domain-containing protein	-
J0G24_RS14665 (3000558)	3000558..3001034	-	477	WP_001439314.1	RadC family protein	-
J0G24_RS14670 (3001050)	3001050..3001520	-	471	WP_016232931.1	antirestriction protein	-
J0G24_RS14675 (3001614)	3001614..3001859	-	246	WP_001164966.1	hypothetical protein	-
J0G24_RS14680 (3001859)	3001859..3002680	-	822	WP_044694629.1	DUF932 domain-containing protein	-
J0G24_RS14685 (3002784)	3002784..3002972	+	189	WP_001291444.1	hypothetical protein	-
J0G24_RS14690 (3003091)	3003091..3003546	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 7.8044

>T196050 WP_042022646.1 NZ_CP071259:c2999140-2998763 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMVRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T196050 NZ_CP095020:c754037-753729 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT196050 WP_001285482.1 NZ_CP071259:c2999561-2999187 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT196050 NZ_CP095020:c754269-754024 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0P7R0L9