Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2634993..2635631 Replicon chromosome
Accession NZ_CP071259
Organism Escherichia coli strain MEI005

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J0G24_RS12800 Protein ID WP_000813794.1
Coordinates 2635455..2635631 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J0G24_RS12795 Protein ID WP_001270286.1
Coordinates 2634993..2635409 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G24_RS12775 (2630145) 2630145..2631086 - 942 WP_001251315.1 ABC transporter permease -
J0G24_RS12780 (2631087) 2631087..2632100 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
J0G24_RS12785 (2632118) 2632118..2633263 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
J0G24_RS12790 (2633508) 2633508..2634914 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
J0G24_RS12795 (2634993) 2634993..2635409 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J0G24_RS12800 (2635455) 2635455..2635631 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J0G24_RS12805 (2635853) 2635853..2636083 + 231 WP_000494244.1 YncJ family protein -
J0G24_RS12810 (2636175) 2636175..2638136 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J0G24_RS12815 (2638209) 2638209..2638745 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
J0G24_RS12820 (2638798) 2638798..2640012 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2640052..2641200 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T196049 WP_000813794.1 NZ_CP071259:c2635631-2635455 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T196049 NZ_CP095020:c752411-752028 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT196049 WP_001270286.1 NZ_CP071259:c2635409-2634993 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT196049 NZ_CP095020:c752661-752506 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References