Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1445026..1445651 Replicon chromosome
Accession NZ_CP071259
Organism Escherichia coli strain MEI005

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag J0G24_RS07075 Protein ID WP_000911329.1
Coordinates 1445253..1445651 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag J0G24_RS07070 Protein ID WP_000450524.1
Coordinates 1445026..1445253 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G24_RS07045 (1440828) 1440828..1441298 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
J0G24_RS07050 (1441298) 1441298..1441870 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
J0G24_RS07055 (1442016) 1442016..1442894 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
J0G24_RS07060 (1442911) 1442911..1443945 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
J0G24_RS07065 (1444158) 1444158..1444871 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
J0G24_RS07070 (1445026) 1445026..1445253 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
J0G24_RS07075 (1445253) 1445253..1445651 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
J0G24_RS07080 (1445798) 1445798..1446661 + 864 WP_001267498.1 neutral zinc metallopeptidase -
J0G24_RS07085 (1446676) 1446676..1448691 + 2016 WP_000829293.1 tRNA cytosine(34) acetyltransferase TmcA -
J0G24_RS07090 (1448765) 1448765..1449463 + 699 WP_000679823.1 esterase -
J0G24_RS07095 (1449573) 1449573..1449773 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T196037 WP_000911329.1 NZ_CP071259:1445253-1445651 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T196037 NZ_CP095020:361900-362202 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT196037 WP_000450524.1 NZ_CP071259:1445026-1445253 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT196037 NZ_CP095020:361676-361903 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGAGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References