Detailed information of TA system TA196034

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 900545..901380
• Replicon: chromosome
• Accession: NZ_CP071259
• Organism: Escherichia coli strain MEI005

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: J0G24_RS04435
• Protein ID: WP_075370645.1
• Coordinates: 900545..900922 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L4J164
• Locus tag: J0G24_RS04440
• Protein ID: WP_001406130.1
• Coordinates: 901012..901380 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G24_RS04410 (896438)	896438..896974	+	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
J0G24_RS04415 (897309)	897309..898844	-	1536	WP_075370643.1	EAL domain-containing protein	-
J0G24_RS04420 (898915)	898915..899760	-	846	WP_075370644.1	DUF4942 domain-containing protein	-
J0G24_RS04425 (899845)	899845..900042	-	198	WP_000839267.1	DUF957 domain-containing protein	-
J0G24_RS04430 (900059)	900059..900548	-	490	Protein_873	DUF5983 family protein	-
J0G24_RS04435 (900545)	900545..900922	-	378	WP_075370645.1	TA system toxin CbtA family protein	Toxin
J0G24_RS04440 (901012)	901012..901380	-	369	WP_001406130.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J0G24_RS04445 (901543)	901543..901764	-	222	WP_000692345.1	DUF987 domain-containing protein	-
J0G24_RS04450 (901839)	901839..902315	-	477	WP_075370646.1	RadC family protein	-
J0G24_RS04455 (902331)	902331..902816	-	486	WP_075370647.1	antirestriction protein	-
J0G24_RS04460 (902908)	902908..903726	-	819	WP_075370648.1	DUF932 domain-containing protein	-
J0G24_RS04465 (904066)	904066..905136	-	1071	WP_075370649.1	patatin-like phospholipase family protein	-
J0G24_RS04470 (905133)	905133..906038	-	906	WP_075370650.1	diguanylate cyclase regulator RdcB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14216.18 Da
• Isoelectric Point: 7.3526

>T196034 WP_075370645.1 NZ_CP071259:c900922-900545 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYDLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSVDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T196034 NZ_CP095011:c3835324-3834902 [Streptomyces sp. 2P-4]
GTGAACGCGGCGCTCTACCTGATCGACACCAGCGCGCTCGCCCGCTTCATGCGGGGCGACGCGGAGCAGTACGGCTGGGA
CCAGGCGGCAGCCGCCGGCCTGATCGCCACGTGCCCCGTCACGGAGCTGGAGTTCTTCTACAGCGCCCGGTCGGCAGCCG
ACCGGGCACGCGGCATTGAGGACATGCGGTTGCTCTTCGGCTGGGTTCCCGTCGACGACCGCGCCTACGACCGCGCCTGG
CAGGTCCAGGAGGCCCTCACCAAGCAAGGCAAGCACCGCAGCGCAGGTGCGGTCGACCTCGTCGTCGCAGCGACTGCCGA
GCTGCAGGGGCTCACCCTCCTGCACTGTGACCACGACTTCGACTGCATCGCCGCAGTAACCGGCCAGCCCGTTCAGTGGT
ACGGCCCTGAGGGCCGTAAGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13717.53 Da
• Isoelectric Point: 7.0269

>AT196034 WP_001406130.1 NZ_CP071259:c901380-901012 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT196034 NZ_CP095011:c3835536-3835321 [Streptomyces sp. 2P-4]
ATGAGTCGTACGGTGATCGACCTCGATGACGAACTGGTCGCGGACGTGGCCAAGGCTCTGGGGACCAGCACCAAGAAGGA
AACGGTCAACACCGCCTTGCGCGAGGTGCTGGAGAACAGGCGGCGCGCGCTGGCCCTGACCCGCCTGCGCGCCGCGGCCG
ACGAGGGCTCATTCGATCTGACGCTCTTCGAGGACAAGCGGAACTACCGGCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1L4J164