Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 379251..379882 Replicon chromosome
Accession NZ_CP071259
Organism Escherichia coli strain MEI005

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag J0G24_RS01815 Protein ID WP_001259388.1
Coordinates 379251..379526 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J0G24_RS01820 Protein ID WP_000593555.1
Coordinates 379523..379882 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G24_RS01800 (374255) 374255..375322 + 1068 WP_000361467.1 HlyD family secretion protein -
J0G24_RS01805 (375319) 375319..378054 + 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
J0G24_RS01810 (378054) 378054..379178 + 1125 WP_001216257.1 ABC transporter permease -
J0G24_RS01815 (379251) 379251..379526 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
J0G24_RS01820 (379523) 379523..379882 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J0G24_RS01825 (380002) 380002..380403 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J0G24_RS01830 (380409) 380409..381215 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
J0G24_RS01835 (381212) 381212..381976 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J0G24_RS01840 (381976) 381976..382809 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J0G24_RS01845 (382806) 382806..383750 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 372267..380403 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T196032 WP_001259388.1 NZ_CP071259:379251-379526 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T196032 NZ_CP095011:2620434-2620697 [Streptomyces sp. 2P-4]
GTGAGTGAGTACCGAACCGCCTTCCGCCCCGAGGCGCAGGCCGAGCTTCGGAAGATCCCCCGCGACGTGGCGCTGCGCAT
CCTGGCCAAGCTGACCGAGCTGGAGACCGACCCTCTCGGCTTCAACACCACCGCACTCGCGTCACAGCCGGAACGACGCC
GTCTTCGCGTCGGCGACTACCGCGTCGTCTACACGATCGACAACGGCGAGTTGGCGATCTGGGTCGTTCACGTGGGACAC
CGGTCCACCGTTTACGAGACCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT196032 WP_000593555.1 NZ_CP071259:379523-379882 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT196032 NZ_CP095011:2620172-2620441 [Streptomyces sp. 2P-4]
ATGACGCAGCCACTGCCCATAGAGTCCATTCGCGACGTGCGAGCACACCTGGCGGAGGTCGTGGAGCGCGCAGACCGCGA
CGATGTACCCACAGTGATCACGCGCCGAGGCAAGGAAGTCGCGGCCGTCGTCTCCATTGAGGTGCTGCGCAGGTACCAGG
AGTGGGAAGAGCGCGAGATCAATCGGATCATCGACGAGCGCATGGCCAACCCAGCACCCGGCATCCCGATCGAGGACATC
ATGAGGGAGACGCTGGCGCGCGGTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References