Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53667..54403 Replicon plasmid p1_IncFIB(K)
Accession NZ_CP071253
Organism Klebsiella pneumoniae strain MEI006

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag J0G26_RS26295 Protein ID WP_003026803.1
Coordinates 53921..54403 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag J0G26_RS26290 Protein ID WP_003026799.1
Coordinates 53667..53933 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G26_RS26245 (49729) 49729..50091 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
J0G26_RS26250 (50141) 50141..50491 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
J0G26_RS26255 (50849) 50849..51118 + 270 WP_004152102.1 hypothetical protein -
J0G26_RS26260 (51106) 51106..51681 + 576 WP_004152103.1 hypothetical protein -
J0G26_RS26265 (51712) 51712..52206 + 495 WP_004152104.1 DNA-binding protein -
J0G26_RS26270 (52250) 52250..52618 + 369 WP_004152105.1 hypothetical protein -
J0G26_RS26275 (52652) 52652..52855 + 204 WP_004152106.1 HHA domain-containing protein -
J0G26_RS26280 (52904) 52904..53161 + 258 WP_004152107.1 hypothetical protein -
J0G26_RS26285 (53237) 53237..53491 + 255 WP_004152108.1 hypothetical protein -
J0G26_RS26290 (53667) 53667..53933 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
J0G26_RS26295 (53921) 53921..54403 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
J0G26_RS26300 (54615) 54615..55961 + 1347 WP_020314316.1 ISNCY family transposase -
J0G26_RS28315 (56122) 56122..56253 + 132 WP_004218042.1 hypothetical protein -
J0G26_RS26305 (56462) 56462..57627 - 1166 Protein_59 IS3 family transposase -
J0G26_RS26310 (57804) 57804..58766 - 963 WP_004152113.1 zinc metalloprotease HtpX -
J0G26_RS26315 (58753) 58753..59241 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(D) / blaSHV-1 - 1..190768 190768


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T196029 WP_003026803.1 NZ_CP071253:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T196029 NZ_CP095003:6385-6801 [Klebsiella michiganensis]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT196029 WP_003026799.1 NZ_CP071253:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT196029 NZ_CP095003:6158-6388 [Klebsiella michiganensis]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References