Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5294559..5295184 Replicon chromosome
Accession NZ_CP071252
Organism Klebsiella pneumoniae strain MEI006

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J0G26_RS25650 Protein ID WP_002882817.1
Coordinates 5294559..5294942 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J0G26_RS25655 Protein ID WP_004150355.1
Coordinates 5294942..5295184 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G26_RS25635 (5291925) 5291925..5292827 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J0G26_RS25640 (5292824) 5292824..5293459 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J0G26_RS25645 (5293456) 5293456..5294385 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J0G26_RS25650 (5294559) 5294559..5294942 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J0G26_RS25655 (5294942) 5294942..5295184 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J0G26_RS25660 (5295389) 5295389..5296306 + 918 WP_009484979.1 alpha/beta hydrolase -
J0G26_RS25665 (5296320) 5296320..5297261 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J0G26_RS25670 (5297306) 5297306..5297743 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J0G26_RS25675 (5297740) 5297740..5298600 - 861 WP_004146232.1 virulence factor BrkB family protein -
J0G26_RS25680 (5298594) 5298594..5299193 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T196028 WP_002882817.1 NZ_CP071252:c5294942-5294559 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T196028 NZ_CP095003:c5310-5005 [Klebsiella michiganensis]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAGCTCTATCCGGTGG
TGCACATCGGGGACGAGAGCTGGCGCCTGATGACCACGGACATGGCCAGCGTCCCGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCCGCCACCGGGAAAATGACATCAAAAACGCAATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT196028 WP_004150355.1 NZ_CP071252:c5295184-5294942 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT196028 NZ_CP095003:c5530-5312 [Klebsiella michiganensis]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGCTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References