Detailed information of TA system TA196027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4974963..4976644
• Replicon: chromosome
• Accession: NZ_CP071252
• Organism: Klebsiella pneumoniae strain MEI006

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: J0G26_RS24175
• Protein ID: WP_004146663.1
• Coordinates: 4974963..4975880 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: J0G26_RS24180
• Protein ID: WP_004177809.1
• Coordinates: 4975886..4976644 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G26_RS24155 (4970269)	4970269..4971789	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
J0G26_RS24160 (4971786)	4971786..4972784	+	999	WP_002885172.1	ribose ABC transporter permease	-
J0G26_RS24165 (4972815)	4972815..4973756	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
J0G26_RS24170 (4973831)	4973831..4974838	+	1008	WP_009484222.1	carbohydrate kinase family protein	-
J0G26_RS24175 (4974963)	4974963..4975880	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
J0G26_RS24180 (4975886)	4975886..4976644	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
J0G26_RS24185 (4976730)	4976730..4977458	-	729	WP_002885152.1	response regulator transcription factor	-
J0G26_RS24190 (4977731)	4977731..4979878	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
J0G26_RS24195 (4980340)	4980340..4981026	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T196027 WP_004146663.1 NZ_CP071252:c4975880-4974963 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T196027 NZ_CP095001:c64601-64314 [Klebsiella michiganensis]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAGGAATGGCAAAAGCTGGATAAGGCCATTCAGCAACAGTTTGC
GAAGAAGCTGAAGAAGTGCTGTGACGAACCACATATCCCCTCAGCAAAGCTGAGAGGGATAAAGGACTGCTATAAAATTA
AACTTCGCGCGTCAGGCTTTCGTCTGGTCTATCAGGTAATAGATGATCAACTGATTATTGCTGTTGTGGCTGTCGGAAAG
CGTGAACGTAGTGACGTGTACAATCTGGCCAGCGAACGGATGAGGTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT196027 WP_004177809.1 NZ_CP071252:c4976644-4975886 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT196027 NZ_CP095001:c64849-64598 [Klebsiella michiganensis]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATCAGCGAACTTAAAAAAAACCCGATGGCGACGGTCAGTGCCGG
CGACGGCTTCCCGGTCGCTATTCTGAACCGTAATCAGCCCGCTTTTTACTGCATCCCGGCAGAGCTTTACGAGAAGATGC
TCGATGCCCTGGATGATCAGGAACTGGCAAAGCTGGTTTCAGAACGCAGTAACCAGCGACTGCTTGACGTGGATCTGGAC
ACATATCTATGA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05