Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4698967..4699777 Replicon chromosome
Accession NZ_CP071252
Organism Klebsiella pneumoniae strain MEI006

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag J0G26_RS22780 Protein ID WP_004178461.1
Coordinates 4698967..4699500 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag J0G26_RS22785 Protein ID WP_002887278.1
Coordinates 4699511..4699777 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G26_RS22775 (4697798) 4697798..4698919 + 1122 WP_002887282.1 cupin domain-containing protein -
J0G26_RS22780 (4698967) 4698967..4699500 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
J0G26_RS22785 (4699511) 4699511..4699777 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
J0G26_RS22790 (4699880) 4699880..4701313 - 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
J0G26_RS22795 (4701303) 4701303..4701986 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
J0G26_RS22800 (4702158) 4702158..4703543 + 1386 WP_009484676.1 efflux transporter outer membrane subunit -
J0G26_RS22805 (4703561) 4703561..4703905 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T196025 WP_004178461.1 NZ_CP071252:c4699500-4698967 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T196025 NZ_CP094999:c5426667-5426278 [Klebsiella michiganensis]
ATGCAAACAAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGTGATGAGCAGGTCATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGTTGATGCCCTGAATTTTATCGTTGAGAAGTATGAACTGGTCCGCACTGACCGCCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGGAAAGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGCGCTCCAGGCAAGCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT196025 WP_002887278.1 NZ_CP071252:c4699777-4699511 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT196025 NZ_CP094999:c5427082-5426723 [Klebsiella michiganensis]
TTGTCGAAGAACACCACGATAACAACGCATGATATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTT
CGGTGCCCGTCTGGTCCAGGAGGGAAACCGCCTGCATTACCTGGCTGACCGGGCGAATGTTAGCGGGCAGTTCTGTGATG
CGGATTTACGCCATCTTGACCAGGCTTTCCCGGTATTGATGAAACAGCTGGAGTTAATGCTCACCTGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCACGTTGTATGCAAAAGGTCTGACCTGCGAGGCTGATACGCTGGGTTCGCACGGCTATGT
TTATATCGCGATTTATCCCACTCCCGTCGCCACGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References