Detailed information of TA system TA196013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 325295..325881
• Replicon: chromosome
• Accession: NZ_CP071252
• Organism: Klebsiella pneumoniae strain MEI006

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: J0G26_RS01505
• Protein ID: WP_002920800.1
• Coordinates: 325513..325881 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: J0G26_RS01500
• Protein ID: WP_004174006.1
• Coordinates: 325295..325516 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G26_RS01480 (321452)	321452..322378	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
J0G26_RS01485 (322375)	322375..323652	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
J0G26_RS01490 (323649)	323649..324416	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
J0G26_RS01495 (324418)	324418..325131	+	714	WP_009484078.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
J0G26_RS01500 (325295)	325295..325516	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J0G26_RS01505 (325513)	325513..325881	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
J0G26_RS01510 (326154)	326154..327470	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
J0G26_RS01515 (327577)	327577..328464	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
J0G26_RS01520 (328461)	328461..329306	+	846	WP_004174009.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
J0G26_RS01525 (329308)	329308..330378	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	320281..333906	13625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T196013 WP_002920800.1 NZ_CP071252:325513-325881 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T196013 NZ_CP094998:3345833-3346195 [Synechocystis sp. PCC 6803]
ATGTCGAATAATCTCCATTTAGTCAATATCGACTTCACCCCCGAATATAGGAGAAGTTTAAAATATTTAGCCAAGAAATA
TCGAAATATTCGTTCTGATGTTCAGCCCATTATTGAGGCTTTGCAAAAAGGCGTCATTAGTGGTGATCGCCTGGCAGGGT
TTGGCTCTGATATTTACGTCTATAAGCTTAGAATTAAGAATAGTAATATTCAGAAAGGGAAAAGTTCTGGCTACCGATTA
ATTTATTTACTCGAATCAGAAAACAGTATTTTGTTGTTAACCATTTACAGTAAAGCTGAACAAGAAGATATTGCCGCTAG
TGACATTAATTCAATTTTAGGGGAGTACTCCATAGAAGACTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT196013 WP_004174006.1 NZ_CP071252:325295-325516 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT196013 NZ_CP094998:3345583-3345843 [Synechocystis sp. PCC 6803]
ATGATGAGAGCTTTTGAGGTAATGGCCACAGTTAAAGATTCCAAACAATTGCTCTTGGATAGTGATTTGCATTGGAATAC
CAGCCGAGTCAAGGTGATTATTTTGGAATCAGATGAATTGGCATCAAAAGGAAGTGAGTTTGATCCTGATGACACGCCTG
TCGAAGAAATTAAGGTGAGTTTGAGGAAGGCATTAGAGGAATATAAACAAGGAAAAAGAATTCCAGTAGAAAATATGTGG
GAGGGAATTGATGTCGAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7