Detailed information of TA system TA196010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 2105201..2105756
• Replicon: chromosome
• Accession: NZ_CP071248
• Organism: Bifidobacterium longum subsp. infantis strain JCM 7009

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BLI009_RS09310
• Protein ID: WP_206648514.1
• Coordinates: 2105433..2105756 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D6ZTS2
• Locus tag: BLI009_RS09305
• Protein ID: WP_013140588.1
• Coordinates: 2105201..2105446 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLI009_RS09285	2100615..2100800	-	186	WP_206648512.1	hypothetical protein	-
BLI009_RS09290	2101262..2101924	+	663	WP_131268475.1	TetR/AcrR family transcriptional regulator	-
BLI009_RS09295	2102081..2103352	+	1272	WP_206648513.1	MFS transporter	-
BLI009_RS09300	2103773..2105029	-	1257	WP_019728042.1	HipA domain-containing protein	-
BLI009_RS09305	2105201..2105446	+	246	WP_013140588.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLI009_RS09310	2105433..2105756	+	324	WP_206648514.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLI009_RS09315	2105973..2106587	-	615	WP_003829107.1	cupin domain-containing protein	-
BLI009_RS09320	2106682..2107551	-	870	WP_003829104.1	class I SAM-dependent methyltransferase	-
BLI009_RS09325	2107789..2108154	+	366	WP_013140585.1	YccF domain-containing protein	-
BLI009_RS09330	2108698..2109963	+	1266	WP_206648515.1	Fic family protein	-
BLI009_RS09335	2110433..2110731	+	299	Protein_1804	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11831.61 Da
• Isoelectric Point: 4.7434

>T196010 WP_206648514.1 NZ_CP071248:2105433-2105756 [Bifidobacterium longum subsp. infantis]
MKRGEIWTLQADGYASKPQPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Nucleotide

• Length: 324 bp

>T196010 NZ_CP094998:c1932672-1932286 [Synechocystis sp. PCC 6803]
ATGAATTCTGTTTTGATAGACACCCACGTTTTTATTTGGTTAGCAGAAGATGACTCTAACTTGCCTGTGACGACAAAAGA
TACCTTAGAAAATATGGATAACGTTTTTGTTAGTATTGCCAGCTTTTGGGAAATTTCTATTAAACTAAAAATTGGTAAAC
TTTCTCTCTGTGGTGATTTCAATAGTATTGAAGCTAGTTTTCAAAGAACCCGATTCAAGCTATTACCTATTACACTAAAA
GACACAATTAAACTTTATAATCTTCCCTTGCACCACAAAGATCCCTTTGATCGTATTTTGGTATCCCAATCGATTAATCA
TTCTCTTATTTTAGTCAGTCGTGATCAAGCTCTGGATGATTATCCGATTCAACGCCTGTGGTTGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9293.39 Da
• Isoelectric Point: 4.7719

>AT196010 WP_013140588.1 NZ_CP071248:2105201-2105446 [Bifidobacterium longum subsp. infantis]
MSIQIATRVSDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT196010 NZ_CP094998:c1932878-1932672 [Synechocystis sp. PCC 6803]
ATGACTACTGAAGCTGCCCTATTAAACATTGCTGAAAATCTGCCTGATGACTTAAAAATCGAGCTTTTACACTATGCTGA
ATATTTAATGAATAAATCCTTAAATGCGCCCCAAAAGCCATTAGCAGGATCGATGAAAGGAACTTTTATTTTACCTCTAC
CCGAAGACTTTGATGCCCCCTTAGAAGAATTTGAGGAGTATATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A087BFX1