Detailed information of TA system TA195995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4453981..4454576
• Replicon: chromosome
• Accession: NZ_CP071244
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S37_jyu2015

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: J0F95_RS21250
• Protein ID: WP_071605322.1
• Coordinates: 4454322..4454576 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J0F95_RS21245
• Protein ID: WP_033633573.1
• Coordinates: 4453981..4454322 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F95_RS21225	4449489..4450304	-	816	WP_033639337.1	GPO family capsid scaffolding protein	-
J0F95_RS21230	4450447..4452219	+	1773	WP_033639336.1	terminase ATPase subunit family protein	-
J0F95_RS21235	4452219..4452905	+	687	WP_060445964.1	terminase family protein	-
J0F95_RS21240	4452902..4453936	+	1035	WP_033639335.1	phage portal protein	-
J0F95_RS21245	4453981..4454322	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0F95_RS21250	4454322..4454576	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0F95_RS21255	4454946..4455143	-	198	WP_126186641.1	hypothetical protein	-
J0F95_RS21260	4455191..4455415	-	225	WP_033639333.1	hypothetical protein	-
J0F95_RS21265	4455454..4457670	-	2217	WP_033639332.1	replication endonuclease	-
J0F95_RS21270	4457667..4457981	-	315	WP_033639331.1	DUF3850 domain-containing protein	-
J0F95_RS21275	4457995..4458168	-	174	WP_162531492.1	hypothetical protein	-
J0F95_RS21280	4458108..4458332	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
J0F95_RS21285	4458332..4458574	-	243	WP_023447523.1	DUF2732 family protein	-
J0F95_RS21290	4458638..4458940	-	303	WP_031221632.1	hypothetical protein	-
J0F95_RS21295	4458952..4459131	-	180	WP_072009648.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	4413269..4462169	48900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T195995 WP_071605322.1 NZ_CP071244:c4454576-4454322 [Serratia marcescens subsp. marcescens ATCC 13880]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T195995 NZ_CP094991:c4711333-4710992 [Klebsiella pneumoniae]
ATGAAAACCTTACCTGCAACAATTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCCTGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCGCAGAGGGTTT
AACTGTCAGGAACAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGGCAGGCAACCGGCCTGTTACGGCAAAG
ACATCTTCCTTCGATACGCTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT195995 WP_033633573.1 NZ_CP071244:c4454322-4453981 [Serratia marcescens subsp. marcescens ATCC 13880]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT195995 NZ_CP094991:c4711683-4711357 [Klebsiella pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGGTGACGCCGAATGTCTGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1