Detailed information of TA system TA195867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4453974..4454569
• Replicon: chromosome
• Accession: NZ_CP071228
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S78_jyu2015

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: J0F98_RS21250
• Protein ID: WP_071605322.1
• Coordinates: 4454315..4454569 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J0F98_RS21245
• Protein ID: WP_033633573.1
• Coordinates: 4453974..4454315 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F98_RS21225	4449482..4450297	-	816	WP_033639337.1	GPO family capsid scaffolding protein	-
J0F98_RS21230	4450440..4452212	+	1773	WP_033639336.1	terminase ATPase subunit family protein	-
J0F98_RS21235	4452212..4452898	+	687	WP_060445964.1	terminase family protein	-
J0F98_RS21240	4452895..4453929	+	1035	WP_033639335.1	phage portal protein	-
J0F98_RS21245	4453974..4454315	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0F98_RS21250	4454315..4454569	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0F98_RS21255	4454939..4455136	-	198	WP_126186641.1	hypothetical protein	-
J0F98_RS21260	4455184..4455408	-	225	WP_033639333.1	hypothetical protein	-
J0F98_RS21265	4455447..4457663	-	2217	WP_033639332.1	replication endonuclease	-
J0F98_RS21270	4457660..4457974	-	315	WP_033639331.1	DUF3850 domain-containing protein	-
J0F98_RS21275	4457988..4458161	-	174	WP_162531492.1	hypothetical protein	-
J0F98_RS21280	4458101..4458325	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
J0F98_RS21285	4458325..4458567	-	243	WP_023447523.1	DUF2732 family protein	-
J0F98_RS21290	4458631..4458933	-	303	WP_031221632.1	hypothetical protein	-
J0F98_RS21295	4458945..4459124	-	180	WP_072009648.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(6')-Ic	rfaE	4372060..4489596	117536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T195867 WP_071605322.1 NZ_CP071228:c4454569-4454315 [Serratia marcescens subsp. marcescens ATCC 13880]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T195867 NZ_CP094865:1038974-1039153 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT195867 WP_033633573.1 NZ_CP071228:c4454315-4453974 [Serratia marcescens subsp. marcescens ATCC 13880]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT195867 NZ_CP094865:1038351-1038950 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAATTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1