Detailed information of TA system TA195771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4455249..4455844
• Replicon: chromosome
• Accession: NZ_CP071216
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S24_jyu2015

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: J0G01_RS21260
• Protein ID: WP_071605322.1
• Coordinates: 4455590..4455844 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J0G01_RS21255
• Protein ID: WP_033633573.1
• Coordinates: 4455249..4455590 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G01_RS21235	4450757..4451572	-	816	WP_033639337.1	GPO family capsid scaffolding protein	-
J0G01_RS21240	4451715..4453487	+	1773	WP_033639336.1	terminase ATPase subunit family protein	-
J0G01_RS21245	4453487..4454173	+	687	WP_060445964.1	terminase family protein	-
J0G01_RS21250	4454170..4455204	+	1035	WP_033639335.1	phage portal protein	-
J0G01_RS21255	4455249..4455590	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0G01_RS21260	4455590..4455844	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0G01_RS21265	4456214..4456411	-	198	WP_126186641.1	hypothetical protein	-
J0G01_RS21270	4456459..4456683	-	225	WP_033639333.1	hypothetical protein	-
J0G01_RS21275	4456722..4458938	-	2217	WP_033639332.1	replication endonuclease	-
J0G01_RS21280	4458935..4459249	-	315	WP_033639331.1	DUF3850 domain-containing protein	-
J0G01_RS21285	4459263..4459436	-	174	WP_162531492.1	hypothetical protein	-
J0G01_RS21290	4459376..4459600	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
J0G01_RS21295	4459600..4459842	-	243	WP_023447523.1	DUF2732 family protein	-
J0G01_RS21300	4459906..4460208	-	303	WP_031221632.1	hypothetical protein	-
J0G01_RS21305	4460220..4460399	-	180	WP_072009648.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(6')-Ic	rfaE	4373335..4473424	100089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T195771 WP_071605322.1 NZ_CP071216:c4455844-4455590 [Serratia marcescens subsp. marcescens ATCC 13880]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T195771 NZ_CP094815:854688-855059 [Mammaliicoccus sciuri]
ATGAGAAGAGGAGATGTATATCTCGCAGATCTTTCACCAGTTACAGGTTCTGAACAAGGGGGAACAAGACCAGTCGTTAT
TATTCAGAATGATACAGGTAATCGATACAGCCCGACTGTGATTGTTGCAGCAATTACTGGTAAGATAAATAAAGCCAAAA
TTCCCACTCATGTAGAAATTGAGGCAGCGAAATATAAATTAGACAGAGATTCCGTAATTTTGCTTGAACAAATCAGAACA
ATTGATAAAAAACGCTTAAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATATCGCTATTGCCAT
CAGCCTCAATCTAACTTTGCAACAAAAATTTGATACATTAGGTAATACATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT195771 WP_033633573.1 NZ_CP071216:c4455590-4455249 [Serratia marcescens subsp. marcescens ATCC 13880]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT195771 NZ_CP094815:854520-854687 [Mammaliicoccus sciuri]
ATGACATCTTTAACGCAAAATCGTACACAAAGTTTAGAACAAACACTGAAAGAAGGCTATCGTTCAATGGCCGATTTAAA
TCTCTCCCTTGCATCTGAAGCATACTATTGTGAATGTGAAGCTTGTGATTCAAATGAATCGCATATAGCCTCTCAGCAAG
GTGAATAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1