Detailed information of TA system TA195707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4453993..4454588
• Replicon: chromosome
• Accession: NZ_CP071208
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S81_jyu2015

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: J0F85_RS21245
• Protein ID: WP_071605322.1
• Coordinates: 4454334..4454588 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J0F85_RS21240
• Protein ID: WP_033633573.1
• Coordinates: 4453993..4454334 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F85_RS21220	4449501..4450316	-	816	WP_033639337.1	GPO family capsid scaffolding protein	-
J0F85_RS21225	4450459..4452231	+	1773	WP_033639336.1	terminase ATPase subunit family protein	-
J0F85_RS21230	4452231..4452917	+	687	WP_060445964.1	terminase family protein	-
J0F85_RS21235	4452914..4453948	+	1035	WP_033639335.1	phage portal protein	-
J0F85_RS21240	4453993..4454334	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0F85_RS21245	4454334..4454588	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0F85_RS21250	4454958..4455155	-	198	WP_126186641.1	hypothetical protein	-
J0F85_RS21255	4455203..4455427	-	225	WP_033639333.1	hypothetical protein	-
J0F85_RS21260	4455466..4457682	-	2217	WP_033639332.1	replication endonuclease	-
J0F85_RS21265	4457679..4457993	-	315	WP_033639331.1	DUF3850 domain-containing protein	-
J0F85_RS21270	4458007..4458180	-	174	WP_162531492.1	hypothetical protein	-
J0F85_RS21275	4458120..4458344	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
J0F85_RS21280	4458344..4458586	-	243	WP_023447523.1	DUF2732 family protein	-
J0F85_RS21285	4458650..4458952	-	303	WP_031221632.1	hypothetical protein	-
J0F85_RS21290	4458964..4459143	-	180	WP_072009648.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	4413281..4462181	48900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T195707 WP_071605322.1 NZ_CP071208:c4454588-4454334 [Serratia marcescens subsp. marcescens ATCC 13880]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T195707 NZ_CP094795:c2466916-2466650 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT195707 WP_033633573.1 NZ_CP071208:c4454334-4453993 [Serratia marcescens subsp. marcescens ATCC 13880]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT195707 NZ_CP094795:c2467173-2466916 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATGATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1