Detailed information of TA system TA195659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4454013..4454608
• Replicon: chromosome
• Accession: NZ_CP071202
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S97_jyu2015

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: J0F97_RS21250
• Protein ID: WP_071605322.1
• Coordinates: 4454354..4454608 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J0F97_RS21245
• Protein ID: WP_033633573.1
• Coordinates: 4454013..4454354 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F97_RS21225	4449521..4450336	-	816	WP_033639337.1	GPO family capsid scaffolding protein	-
J0F97_RS21230	4450479..4452251	+	1773	WP_033639336.1	terminase ATPase subunit family protein	-
J0F97_RS21235	4452251..4452937	+	687	WP_060445964.1	terminase family protein	-
J0F97_RS21240	4452934..4453968	+	1035	WP_033639335.1	phage portal protein	-
J0F97_RS21245	4454013..4454354	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0F97_RS21250	4454354..4454608	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0F97_RS21255	4454978..4455175	-	198	WP_126186641.1	hypothetical protein	-
J0F97_RS21260	4455223..4455447	-	225	WP_033639333.1	hypothetical protein	-
J0F97_RS21265	4455486..4457702	-	2217	WP_033639332.1	replication endonuclease	-
J0F97_RS21270	4457699..4458013	-	315	WP_033639331.1	DUF3850 domain-containing protein	-
J0F97_RS21275	4458027..4458200	-	174	WP_162531492.1	hypothetical protein	-
J0F97_RS21280	4458140..4458364	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
J0F97_RS21285	4458364..4458606	-	243	WP_023447523.1	DUF2732 family protein	-
J0F97_RS21290	4458670..4458972	-	303	WP_031221632.1	hypothetical protein	-
J0F97_RS21295	4458984..4459163	-	180	WP_072009648.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	4413301..4462201	48900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T195659 WP_071605322.1 NZ_CP071202:c4454608-4454354 [Serratia marcescens subsp. marcescens ATCC 13880]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T195659 NZ_CP094785:485067-485393 [Staphylococcus aureus]
ATGAATTGGGAAATTAATGATTTGTTTAGCGATTTGAAAGTGTTGAAAGATAGATTCGAAGATTTAAAGGATAATCATGG
TTGGCATTTTGAGGAGTTATATCCACATGAACCAAATCATAACTTAAATAAAGATGAATTAATTAGAGAGGGATTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTCATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGC
AGAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT195659 WP_033633573.1 NZ_CP071202:c4454354-4454013 [Serratia marcescens subsp. marcescens ATCC 13880]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT195659 NZ_CP094785:484855-485064 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTAAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACTGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1