Detailed information of TA system TA195563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4455228..4455823
• Replicon: chromosome
• Accession: NZ_CP071190
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S79_jyu2015

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: J0F91_RS21260
• Protein ID: WP_071605322.1
• Coordinates: 4455569..4455823 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J0F91_RS21255
• Protein ID: WP_033633573.1
• Coordinates: 4455228..4455569 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F91_RS21235	4450736..4451551	-	816	WP_033639337.1	GPO family capsid scaffolding protein	-
J0F91_RS21240	4451694..4453466	+	1773	WP_033639336.1	terminase ATPase subunit family protein	-
J0F91_RS21245	4453466..4454152	+	687	WP_060445964.1	terminase family protein	-
J0F91_RS21250	4454149..4455183	+	1035	WP_033639335.1	phage portal protein	-
J0F91_RS21255	4455228..4455569	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0F91_RS21260	4455569..4455823	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0F91_RS21265	4456193..4456390	-	198	WP_126186641.1	hypothetical protein	-
J0F91_RS21270	4456438..4456662	-	225	WP_033639333.1	hypothetical protein	-
J0F91_RS21275	4456701..4458917	-	2217	WP_033639332.1	replication endonuclease	-
J0F91_RS21280	4458914..4459228	-	315	WP_033639331.1	DUF3850 domain-containing protein	-
J0F91_RS21285	4459242..4459415	-	174	WP_162531492.1	hypothetical protein	-
J0F91_RS21290	4459355..4459579	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
J0F91_RS21295	4459579..4459821	-	243	WP_023447523.1	DUF2732 family protein	-
J0F91_RS21300	4459885..4460187	-	303	WP_031221632.1	hypothetical protein	-
J0F91_RS21305	4460199..4460378	-	180	WP_072009648.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4426634..4463416	36782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T195563 WP_071605322.1 NZ_CP071190:c4455823-4455569 [Serratia marcescens subsp. marcescens ATCC 13880]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T195563 NZ_CP094763:442557-442883 [Staphylococcus aureus]
ATGAATTGGGAAATTAATGATTTGTTTAGCGATTTGAAAGTGTTGAAAGATAGATTCGAAGATTTAAAGGATAATCATGG
TTGGCATTTTGAGGAGTTATATCCACATGAACCAAATCATAACTTAAATAAAGATGAATTAATTAGAGAGGGATTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATCGAA
AAGTTTCATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGC
AGAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT195563 WP_033633573.1 NZ_CP071190:c4455569-4455228 [Serratia marcescens subsp. marcescens ATCC 13880]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT195563 NZ_CP094763:442345-442554 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTAAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACTGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1