Detailed information of TA system TA195547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4454000..4454595
• Replicon: chromosome
• Accession: NZ_CP071188
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S94_jyu2015

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: J0F99_RS21250
• Protein ID: WP_071605322.1
• Coordinates: 4454341..4454595 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J0F99_RS21245
• Protein ID: WP_033633573.1
• Coordinates: 4454000..4454341 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F99_RS21225	4449508..4450323	-	816	WP_033639337.1	GPO family capsid scaffolding protein	-
J0F99_RS21230	4450466..4452238	+	1773	WP_033639336.1	terminase ATPase subunit family protein	-
J0F99_RS21235	4452238..4452924	+	687	WP_060445964.1	terminase family protein	-
J0F99_RS21240	4452921..4453955	+	1035	WP_033639335.1	phage portal protein	-
J0F99_RS21245	4454000..4454341	-	342	WP_033633573.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J0F99_RS21250	4454341..4454595	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
J0F99_RS21255	4454965..4455162	-	198	WP_126186641.1	hypothetical protein	-
J0F99_RS21260	4455210..4455434	-	225	WP_033639333.1	hypothetical protein	-
J0F99_RS21265	4455473..4457689	-	2217	WP_033639332.1	replication endonuclease	-
J0F99_RS21270	4457686..4458000	-	315	WP_033639331.1	DUF3850 domain-containing protein	-
J0F99_RS21275	4458014..4458187	-	174	WP_162531492.1	hypothetical protein	-
J0F99_RS21280	4458127..4458351	-	225	WP_033639330.1	TraR/DksA C4-type zinc finger protein	-
J0F99_RS21285	4458351..4458593	-	243	WP_023447523.1	DUF2732 family protein	-
J0F99_RS21290	4458657..4458959	-	303	WP_031221632.1	hypothetical protein	-
J0F99_RS21295	4458971..4459150	-	180	WP_072009648.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	4413288..4462188	48900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T195547 WP_071605322.1 NZ_CP071188:c4454595-4454341 [Serratia marcescens subsp. marcescens ATCC 13880]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T195547 NZ_CP094758:1551905-1552381 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTGATAAAGCGTGGTTTATCAGATAGAGAAAAGGCAGGAATTCTCTTCGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCTATTTTCAACAATCGCAAGTTCGAAAATTACGCACGT
AGACACGGCTTTATCTCAGCTGTTCCATTACGTGAAATTGTAGAAGCTTATAATTATGGCGTACGTAACTTGTATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATTAATTACTATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAAAAATTATTTGAGGAGGACATTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12399.90 Da
• Isoelectric Point: 4.5789

>AT195547 WP_033633573.1 NZ_CP071188:c4454341-4454000 [Serratia marcescens subsp. marcescens ATCC 13880]
MNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLSP
ERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT195547 NZ_CP094758:1551569-1551892 [Staphylococcus aureus]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAAA
TTGCCAACGAATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGTGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGGGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAA
GTAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1