Detailed information of TA system TA195534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1107830..1108451
• Replicon: chromosome
• Accession: NZ_CP071188
• Organism: Serratia marcescens subsp. marcescens ATCC 13880 substr. Sm_S94_jyu2015

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A8B4G7F9
• Locus tag: J0F99_RS05170
• Protein ID: WP_004940313.1
• Coordinates: 1107830..1108033 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A2X2G5J9
• Locus tag: J0F99_RS05175
• Protein ID: WP_004940312.1
• Coordinates: 1108083..1108451 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0F99_RS05140	1103530..1103868	+	339	WP_004940327.1	P-II family nitrogen regulator	-
J0F99_RS05145	1103904..1105190	+	1287	WP_004940322.1	ammonium transporter AmtB	-
J0F99_RS05150	1105281..1106144	-	864	WP_016928847.1	acyl-CoA thioesterase II	-
J0F99_RS05155	1106375..1106866	+	492	WP_016928846.1	YbaY family lipoprotein	-
J0F99_RS05160	1106931..1107245	-	315	WP_004940317.1	MGMT family protein	-
J0F99_RS05170	1107830..1108033	-	204	WP_004940313.1	hemolysin expression modulator Hha	Toxin
J0F99_RS05175	1108083..1108451	-	369	WP_004940312.1	Hha toxicity modulator TomB	Antitoxin
J0F99_RS05180	1108610..1108963	-	354	WP_016928845.1	hypothetical protein	-
J0F99_RS05185	1109368..1110081	+	714	WP_033641138.1	ABC transporter ATP-binding protein	-
J0F99_RS05190	1110078..1110935	+	858	WP_004940309.1	metal ABC transporter permease	-
J0F99_RS05195	1110961..1111839	+	879	WP_016928843.1	metal ABC transporter substrate-binding protein	-
J0F99_RS05200	1111947..1112087	-	141	WP_004940304.1	type B 50S ribosomal protein L36	-
J0F99_RS05205	1112100..1112354	-	255	WP_004940303.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8107.37 Da
• Isoelectric Point: 6.9756

>T195534 WP_004940313.1 NZ_CP071188:c1108033-1107830 [Serratia marcescens subsp. marcescens ATCC 13880]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Nucleotide

• Length: 204 bp

>T195534 NZ_CP094755:1994544-1995005 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAGTTCAACAATCGCAAGTTCGAAAATTACGCACGT
AGACACGGCTTTATCTCAGCTGTTCCATTACGCGAAATTGTAGAAGCTTATAATTATGGCGTACGTAACTTGTATGAGTT
GTCCGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAGAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCGATTACATTTGAGCCATTGAGAGTTTATCGATACAAAGAGATATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14261.95 Da
• Isoelectric Point: 4.4314

>AT195534 WP_004940312.1 NZ_CP071188:c1108451-1108083 [Serratia marcescens subsp. marcescens ATCC 13880]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Nucleotide

• Length: 369 bp

>AT195534 NZ_CP094755:1994208-1994531 [Staphylococcus aureus]
ATGAGAACTTCAGCAGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCGAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAAA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGTGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAA
GTAA

Structures

Toxin

• AlphaFold DB: A0A8B4G7F9

Antitoxin

• AlphaFold DB: A0A2X2G5J9