Detailed information of TA system TA195530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 48558..49666
• Replicon: plasmid pLI47-1
• Accession: NZ_CP071180
• Organism: Listeria innocua strain LI47

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: JZX95_RS15105
• Protein ID: WP_000233000.1
• Coordinates: 48558..49427 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: JZX95_RS15110
• Protein ID: WP_000205227.1
• Coordinates: 49442..49666 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZX95_RS15085	44829..46148	-	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
JZX95_RS15090	46376..46903	-	528	WP_002294507.1	phosphoribosyltransferase family protein	-
JZX95_RS15095	46947..47810	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
JZX95_RS15100	47843..48577	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
JZX95_RS15105	48558..49427	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
JZX95_RS15110	49442..49666	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
JZX95_RS15115	49809..50531	-	723	Protein_54	recombinase zinc beta ribbon domain-containing protein	-
JZX95_RS15120	51293..51743	-	451	Protein_55	CatB-related O-acetyltransferase	-
JZX95_RS15125	51777..52310	-	534	WP_193809670.1	GrpB family protein	-
JZX95_RS15130	52435..53898	-	1464	WP_193809671.1	ABC-F type ribosomal protection protein Msr(D)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	ClpL / lnu(A) / cat / tet(S) / ant(6)-Ia / msr(D) / mef(A)	-	1..93516	93516
	-	inside	IScluster/Tn	lnu(A) / cat / tet(S) / ant(6)-Ia / msr(D) / mef(A)	-	31358..55235	23877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T195530 WP_000233000.1 NZ_CP071180:c49427-48558 [Listeria innocua]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T195530 NZ_CP094755:c858936-858748 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT195530 WP_000205227.1 NZ_CP071180:c49666-49442 [Listeria innocua]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT195530 NZ_CP094755:c859225-858965 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: R3JIN1