Detailed information of TA system TA195529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 19071..19648
• Replicon: plasmid pLI47-1
• Accession: NZ_CP071180
• Organism: Listeria innocua strain LI47

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: JZX95_RS14965
• Protein ID: WP_003728465.1
• Coordinates: 19307..19648 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: JZX95_RS14960
• Protein ID: WP_003728464.1
• Coordinates: 19071..19313 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZX95_RS14915	14343..15059	-	717	WP_003731678.1	AP2 domain-containing protein	-
JZX95_RS14920	15075..15797	-	723	WP_003728517.1	CRISPR-associated protein Cas5	-
JZX95_RS14925	15801..16226	-	426	WP_003728518.1	hypothetical protein	-
JZX95_RS14930	16227..16454	-	228	WP_003769261.1	hypothetical protein	-
JZX95_RS15760	16597..16845	-	249	WP_023553717.1	hypothetical protein	-
JZX95_RS14940	17244..17621	-	378	WP_003769263.1	DUF3850 domain-containing protein	-
JZX95_RS14945	17640..18302	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
JZX95_RS14950	18302..18628	-	327	WP_003728462.1	hypothetical protein	-
JZX95_RS14955	18774..19010	+	237	WP_003769265.1	hypothetical protein	-
JZX95_RS14960	19071..19313	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JZX95_RS14965	19307..19648	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JZX95_RS14970	19841..21976	-	2136	WP_003728466.1	heavy metal translocating P-type ATPase	-
JZX95_RS14975	21976..22335	-	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
JZX95_RS14980	22615..23169	+	555	WP_045554658.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	ClpL / lnu(A) / cat / tet(S) / ant(6)-Ia / msr(D) / mef(A)	-	1..93516	93516

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.74 Da
• Isoelectric Point: 9.2830

>T195529 WP_003728465.1 NZ_CP071180:19307-19648 [Listeria innocua]
MVKQGDIIKINLNPKQGHEQQGYRPYICLSHHLVSDYANIAIFAPISNTPRTYPLYVPLTGTTTTGKVLLDQLVTIDYNA
RKHQYVETVSDPLLTKLLDTVKVIFQKNERSTN

Nucleotide

• Length: 342 bp

>T195529 NZ_CP094755:356780-356956 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9207.48 Da
• Isoelectric Point: 4.3867

>AT195529 WP_003728464.1 NZ_CP071180:19071-19313 [Listeria innocua]
MTTVSVKKWGNSKAVRLPNNLLNALDIKENDDLKVEVINNQMILTKTAIDLRIEDLFANYQGEPFQTELQTFEPIGDEKW

Nucleotide

• Length: 243 bp

>AT195529 NZ_CP094755:c357118-356944 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGTGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5