Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 873860..874489 Replicon chromosome
Accession NZ_CP071179
Organism Listeria innocua strain LI47

Toxin (Protein)


Gene name pemK Uniprot ID Q92DC7
Locus tag JZX95_RS04215 Protein ID WP_010990666.1
Coordinates 874142..874489 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID H1GC21
Locus tag JZX95_RS04210 Protein ID WP_003761302.1
Coordinates 873860..874138 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZX95_RS04190 869157..870641 + 1485 WP_045554578.1 PH domain-containing protein -
JZX95_RS04195 870792..872171 + 1380 WP_045554579.1 protoporphyrinogen oxidase -
JZX95_RS04200 872173..872529 + 357 WP_003761296.1 holo-ACP synthase -
JZX95_RS04205 872548..873654 + 1107 WP_010990665.1 alanine racemase -
JZX95_RS04210 873860..874138 + 279 WP_003761302.1 CopG family ribbon-helix-helix protein Antitoxin
JZX95_RS04215 874142..874489 + 348 WP_010990666.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JZX95_RS04220 874741..875577 + 837 WP_003771126.1 STAS domain-containing protein -
JZX95_RS04225 875583..875939 + 357 WP_003721457.1 STAS domain-containing protein -
JZX95_RS04230 875942..876352 + 411 WP_010990667.1 anti-sigma regulatory factor -
JZX95_RS04235 876369..877373 + 1005 WP_003771130.1 PP2C family protein-serine/threonine phosphatase -
JZX95_RS04240 877523..877867 + 345 WP_003721460.1 anti sigma b factor antagonist RsbV -
JZX95_RS04245 877851..878324 + 474 WP_003761314.1 anti-sigma B factor RsbW -
JZX95_RS04250 878302..879081 + 780 WP_003761316.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12801.85 Da        Isoelectric Point: 6.4735

>T195528 WP_010990666.1 NZ_CP071179:874142-874489 [Listeria innocua]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMAKVNQALEVSLGVVEF

Download         Length: 348 bp

>T195528 NZ_CP094752:c2532874-2532608 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGATCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10605.13 Da        Isoelectric Point: 4.2980

>AT195528 WP_003761302.1 NZ_CP071179:873860-874138 [Listeria innocua]
VLEKEKRMIISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVEA
EAEDRNISILGG

Download         Length: 279 bp

>AT195528 NZ_CP094752:c2533131-2532874 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E7E4N0

Antitoxin

Source ID Structure
AlphaFold DB H1GC21

References