Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1079131..1079751 Replicon chromosome
Accession NZ_CP071178
Organism Enterobacter kobei strain CDL29

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag JZY03_RS05035 Protein ID WP_014882802.1
Coordinates 1079131..1079349 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag JZY03_RS05040 Protein ID WP_008499288.1
Coordinates 1079377..1079751 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZY03_RS05005 (JZY03_04990) 1075140..1075400 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
JZY03_RS05010 (JZY03_04995) 1075403..1075543 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
JZY03_RS05015 (JZY03_05000) 1075540..1076250 - 711 WP_032637309.1 GNAT family protein -
JZY03_RS05020 (JZY03_05005) 1076352..1077812 + 1461 WP_059372407.1 PLP-dependent aminotransferase family protein -
JZY03_RS05025 (JZY03_05010) 1077784..1078251 - 468 WP_014882800.1 YlaC family protein -
JZY03_RS05030 (JZY03_05015) 1078370..1078921 - 552 WP_014882801.1 maltose O-acetyltransferase -
JZY03_RS05035 (JZY03_05020) 1079131..1079349 - 219 WP_014882802.1 HHA domain-containing protein Toxin
JZY03_RS05040 (JZY03_05025) 1079377..1079751 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
JZY03_RS05045 (JZY03_05030) 1080263..1083409 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit AcrB -
JZY03_RS05050 (JZY03_05035) 1083432..1084625 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T195515 WP_014882802.1 NZ_CP071178:c1079349-1079131 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T195515 NZ_CP094750:c2507016-2506750 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT195515 WP_008499288.1 NZ_CP071178:c1079751-1079377 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT195515 NZ_CP094750:c2507273-2507016 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATGATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References