Detailed information of TA system TA195508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2640380..2640951
• Replicon: chromosome
• Accession: NZ_CP071176
• Organism: Enterococcus faecalis strain L18

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JZP79_RS12810
• Protein ID: WP_002354774.1
• Coordinates: 2640380..2640721 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: JZP79_RS12815
• Protein ID: WP_002354773.1
• Coordinates: 2640721..2640951 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZP79_RS12805	2636395..2640009	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
JZP79_RS12810	2640380..2640721	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JZP79_RS12815	2640721..2640951	-	231	WP_002354773.1	hypothetical protein	Antitoxin
JZP79_RS12820	2641125..2641340	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
JZP79_RS12825	2641479..2642471	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
JZP79_RS12830	2642638..2643276	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
JZP79_RS12835	2643961..2645577	+	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T195508 WP_002354774.1 NZ_CP071176:c2640721-2640380 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T195508 NZ_CP094750:348110-348361 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTGTATAAGAAAGCGCGAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAGAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTGGTCTATAAATATGAGGAGGAACAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT195508 WP_002354773.1 NZ_CP071176:c2640951-2640721 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT195508 NZ_CP094750:348351-348524 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCCGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AAGGGGACGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812