Detailed information of TA system TA195507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1680035..1680728
• Replicon: chromosome
• Accession: NZ_CP071176
• Organism: Enterococcus faecalis strain L18

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: JZP79_RS08270
• Protein ID: WP_002395798.1
• Coordinates: 1680384..1680728 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: JZP79_RS08265
• Protein ID: WP_002364355.1
• Coordinates: 1680035..1680367 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZP79_RS08215	1675287..1676180	-	894	WP_002369907.1	recombinase RecT	-
JZP79_RS08220	1676180..1677124	-	945	WP_002389314.1	YqaJ viral recombinase family protein	-
JZP79_RS08225	1677222..1677449	-	228	WP_002364220.1	hypothetical protein	-
JZP79_RS08230	1677449..1677772	-	324	WP_002369793.1	hypothetical protein	-
JZP79_RS08235	1677816..1678001	-	186	WP_002364222.1	hypothetical protein	-
JZP79_RS08240	1677992..1678186	-	195	WP_002364223.1	hypothetical protein	-
JZP79_RS08245	1678239..1678421	+	183	WP_002364224.1	YegP family protein	-
JZP79_RS08250	1678461..1679186	-	726	WP_174092948.1	ORF6C domain-containing protein	-
JZP79_RS08255	1679225..1679536	-	312	WP_002381719.1	hypothetical protein	-
JZP79_RS08260	1679547..1679723	-	177	WP_002364354.1	helix-turn-helix transcriptional regulator	-
JZP79_RS08265	1680035..1680367	+	333	WP_002364355.1	helix-turn-helix domain-containing protein	Antitoxin
JZP79_RS08270	1680384..1680728	+	345	WP_002395798.1	ImmA/IrrE family metallo-endopeptidase	Toxin
JZP79_RS08275	1680762..1681490	+	729	WP_002364358.1	ion transporter	-
JZP79_RS08280	1681587..1682735	+	1149	WP_002364359.1	site-specific integrase	-
JZP79_RS08285	1682763..1683206	-	444	WP_002384345.1	hypothetical protein	-
JZP79_RS08290	1683203..1683478	-	276	WP_002356991.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
JZP79_RS08295	1683478..1684524	-	1047	WP_002369171.1	type II secretion system F family protein	-
JZP79_RS08300	1684481..1685449	-	969	WP_002364362.1	Flp pilus assembly complex ATPase component TadA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	efaA	1631505..1837908	206403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (22-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13718.69 Da
• Isoelectric Point: 6.1363

>T195507 WP_002395798.1 NZ_CP071176:1680384-1680728 [Enterococcus faecalis]
MKSIKELVEEYEVELVFAPINKRACYEPVKRIIFVNQNLSIEEQEESIFHEFKHVVSHSDYMELYKIPSFRNKMEAEADH
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLK

Nucleotide

• Length: 345 bp

>T195507 NZ_CP094747:c2510174-2509908 [Staphylococcus aureus]
ATGGTTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTAATTAAAAGTATTGACAGAAATGGTGCGTTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12691.16 Da
• Isoelectric Point: 4.6307

>AT195507 WP_002364355.1 NZ_CP071176:1680035-1680367 [Enterococcus faecalis]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLDEV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT195507 NZ_CP094747:c2510425-2510174 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATAGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTGTAACGAAAGAT
ATAGATGTATAA