Detailed information of TA system TA195500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 89889..90460
• Replicon: chromosome
• Accession: NZ_CP071172
• Organism: Enterococcus faecalis strain L13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JZP77_RS00390
• Protein ID: WP_002354774.1
• Coordinates: 90119..90460 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: JZP77_RS00385
• Protein ID: WP_002354773.1
• Coordinates: 89889..90119 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZP77_RS00365	85263..86879	-	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-
JZP77_RS00370	87564..88202	+	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
JZP77_RS00375	88369..89361	-	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
JZP77_RS00380	89500..89715	+	216	WP_002354768.1	zinc ribbon domain-containing protein	-
JZP77_RS00385	89889..90119	+	231	WP_002354773.1	hypothetical protein	Antitoxin
JZP77_RS00390	90119..90460	+	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JZP77_RS00395	90831..94445	+	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T195500 WP_002354774.1 NZ_CP071172:90119-90460 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T195500 NZ_CP094745:c2154689-2154327 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT195500 WP_002354773.1 NZ_CP071172:89889-90119 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT195500 NZ_CP094745:c2154856-2154686 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812