Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 961913..962542 Replicon chromosome
Accession NZ_CP071157
Organism Listeria innocua strain LI203

Toxin (Protein)


Gene name pemK Uniprot ID Q92DC7
Locus tag JZX96_RS04885 Protein ID WP_010990666.1
Coordinates 962195..962542 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID H1GC21
Locus tag JZX96_RS04880 Protein ID WP_003761302.1
Coordinates 961913..962191 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZX96_RS04860 957210..958694 + 1485 WP_194319938.1 PH domain-containing protein -
JZX96_RS04865 958845..960224 + 1380 WP_194319937.1 protoporphyrinogen oxidase -
JZX96_RS04870 960226..960582 + 357 WP_003761296.1 holo-ACP synthase -
JZX96_RS04875 960601..961707 + 1107 WP_010990665.1 alanine racemase -
JZX96_RS04880 961913..962191 + 279 WP_003761302.1 CopG family ribbon-helix-helix protein Antitoxin
JZX96_RS04885 962195..962542 + 348 WP_010990666.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JZX96_RS04890 962794..963630 + 837 WP_003771126.1 STAS domain-containing protein -
JZX96_RS04895 963636..963992 + 357 WP_003721457.1 STAS domain-containing protein -
JZX96_RS04900 963995..964405 + 411 WP_010990667.1 anti-sigma regulatory factor -
JZX96_RS04905 964422..965426 + 1005 WP_003761312.1 PP2C family protein-serine/threonine phosphatase -
JZX96_RS04910 965576..965920 + 345 WP_003721460.1 anti sigma b factor antagonist RsbV -
JZX96_RS04915 965904..966377 + 474 WP_003761314.1 anti-sigma B factor RsbW -
JZX96_RS04920 966355..967134 + 780 WP_010990669.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12801.85 Da        Isoelectric Point: 6.4735

>T195495 WP_010990666.1 NZ_CP071157:962195-962542 [Listeria innocua]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMAKVNQALEVSLGVVEF

Download         Length: 348 bp

>T195495 NZ_CP094743:c2138229-2138020 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10605.13 Da        Isoelectric Point: 4.2980

>AT195495 WP_003761302.1 NZ_CP071157:961913-962191 [Listeria innocua]
VLEKEKRMIISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVEA
EAEDRNISILGG

Download         Length: 279 bp

>AT195495 NZ_CP094743:c2138534-2138241 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E7E4N0

Antitoxin

Source ID Structure
AlphaFold DB H1GC21

References