Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4587054..4587679 Replicon chromosome
Accession NZ_CP071150
Organism Klebsiella pneumoniae strain IRQBAS102c

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JZ790_RS22270 Protein ID WP_002882817.1
Coordinates 4587296..4587679 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JZ790_RS22265 Protein ID WP_004150355.1
Coordinates 4587054..4587296 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZ790_RS22240 4583045..4583644 + 600 WP_004151865.1 glucose-1-phosphatase -
JZ790_RS22245 4583638..4584498 + 861 WP_004146232.1 virulence factor BrkB family protein -
JZ790_RS22250 4584495..4584932 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JZ790_RS22255 4584977..4585918 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JZ790_RS22260 4585932..4586849 - 918 WP_117260249.1 alpha/beta hydrolase -
JZ790_RS22265 4587054..4587296 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JZ790_RS22270 4587296..4587679 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JZ790_RS22275 4587853..4588782 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JZ790_RS22280 4588779..4589414 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JZ790_RS22285 4589411..4590313 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T195492 WP_002882817.1 NZ_CP071150:4587296-4587679 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T195492 NZ_CP094743:1584291-1584752 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAACAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCATTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT195492 WP_004150355.1 NZ_CP071150:4587054-4587296 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT195492 NZ_CP094743:1583955-1584278 [Staphylococcus aureus]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAAAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATACGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAAA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAACCAGAAAACGAAGTACAACATCGTGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCTGATTATATAAGAAGCAAACGTAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References