Detailed information of TA system TA195477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1622484..1623074
• Replicon: chromosome
• Accession: NZ_CP071150
• Organism: Klebsiella pneumoniae strain IRQBAS102c

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: JZ790_RS07840
• Protein ID: WP_023341911.1
• Coordinates: 1622742..1623074 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: JZ790_RS07835
• Protein ID: WP_000288812.1
• Coordinates: 1622484..1622741 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZ790_RS07810	1618319..1618561	-	243	WP_048299819.1	hypothetical protein	-
JZ790_RS07815	1618828..1620576	+	1749	WP_096106564.1	hypothetical protein	-
JZ790_RS07820	1620655..1621203	+	549	WP_117259920.1	hypothetical protein	-
JZ790_RS07825	1621486..1621946	+	461	Protein_1529	hypothetical protein	-
JZ790_RS07830	1621943..1622149	+	207	WP_117259919.1	helix-turn-helix domain-containing protein	-
JZ790_RS07835	1622484..1622741	+	258	WP_000288812.1	antitoxin	Antitoxin
JZ790_RS07840	1622742..1623074	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JZ790_RS07850	1623396..1624832	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
JZ790_RS07860	1625198..1626652	-	1455	WP_004148975.1	AMP nucleosidase	-
JZ790_RS07865	1626782..1627027	-	246	WP_023284006.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1620655..1632596	11941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T195477 WP_023341911.1 NZ_CP071150:1622742-1623074 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T195477 NZ_CP094738:901112-901333 [Staphylococcus delphini]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGAGTGTTGTTATTGATTTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCATATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT195477 WP_000288812.1 NZ_CP071150:1622484-1622741 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT195477 NZ_CP094738:900812-901099 [Staphylococcus delphini]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAG
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGTTGCATCATGCATAAGATTGACGCAGGGCACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACGTTTCAAAAACTCATGAAAGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0