Detailed information of TA system TA195471

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 2021625..2023053
• Replicon: chromosome
• Accession: NZ_CP071145
• Organism: Streptococcus equi subsp. equi strain 470_007

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: B6YPW2
• Locus tag: JZI48_RS09790
• Protein ID: WP_012679622.1
• Coordinates: 2022211..2023053 (+)
• Length: 281 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: B6YPW1
• Locus tag: JZI48_RS09785
• Protein ID: WP_012679623.1
• Coordinates: 2021625..2022218 (+)
• Length: 198 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZI48_RS09770 (JZI48_09770)	2017343..2019631	+	2289	WP_012679626.1	CHAP domain-containing protein	-
JZI48_RS09775 (JZI48_09775)	2019644..2019931	+	288	WP_012679625.1	conjugal transfer protein	-
JZI48_RS09780 (JZI48_09780)	2019990..2021531	+	1542	Protein_1887	DUF4366 domain-containing protein	-
JZI48_RS09785 (JZI48_09785)	2021625..2022218	+	594	WP_012679623.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JZI48_RS09790 (JZI48_09790)	2022211..2023053	+	843	WP_012679622.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JZI48_RS09795 (JZI48_09795)	2023139..2024479	+	1341	Protein_1890	DNA topoisomerase	-
JZI48_RS09800 (JZI48_09800)	2024483..2025034	+	552	WP_012679620.1	hypothetical protein	-
JZI48_RS09805 (JZI48_09805)	2025076..2025822	+	747	WP_050315911.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1998426..2218002	219576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-268)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (6-51)

Sequences

Toxin

Protein

• Length: 281 a.a.
• Molecular weight: 32683.90 Da
• Isoelectric Point: 5.9694

>T195471 WP_012679622.1 NZ_CP071145:2022211-2023053 [Streptococcus equi subsp. equi]
MNKAKLTALCHKVSKEVGLSFNAVMLYYFLESILKKLAGSKYSERFIFKGGFLLSSVVGIDSRSTVDIDFLLRNMQLSEE
NIVQMLNETLKPEEFDDIFYELQNIVPIKEEDQYGGFRANILCKMENIRQIVPLDIATGDVITPHPIDYKYVSSFGEEEI
IIKAYPIETMLAEKIQTIYARGFLNSRSKDYYDLYIIYKLKDKDVNVEILREACRKTFSYRKTEFDIGKIIDLLEKLKIN
EAFLKRWQAYSRKNLYAKDITFEEVLDNGIKMVEKIKNEN

Nucleotide

• Length: 843 bp

>T195471 NZ_CP094733:2295429-2295890 [Staphylococcus epidermidis]
ATGGGGTTATATGAAAAAATGTTAATAGAACATGATTATATAGAAGTCAGAGAGACAAATGTTATGCCTAATAACCTACA
CGGTCTATGGTTAGGTGATTTAATTTTAATTAAGCGAAACTTATCAGAAGTACGAAAAGCCGAAGTATTATACGAAGAAC
TAGCACACCATAAACTTACATATGGAAACATCTTAGACCAATCTAAATTCAACAACCGTAAATTTGAAAACTACGCTAGG
CGTTACGGATATGAAGCTGCTCTACCTTTGCGCATTCTTGTGGAGGCGCATAACTATGGTGTTAGTAACTTATATGAATT
AGCTGAATATGTTCAATTAAGCGAAGAATATATAGCAGAAATATTGAAACATTACAAAAATAAATATGGTATTGGAACTA
ACTACGGAGAATACTTAATTACATTTGATCCGTTAAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin

Protein

• Length: 198 a.a.
• Molecular weight: 23345.76 Da
• Isoelectric Point: 8.6522

>AT195471 WP_012679623.1 NZ_CP071145:2021625-2022218 [Streptococcus equi subsp. equi]
MNYENKIEKYLKQSGGIITTAYCKENNIPTIYLSRLLKDGRLSKVRKGIYITKDGDYDEYYFFQHQYKKAIFSYETALYL
LGQTDKIPWSIDVTVYNGYKFNEKPNGFNVHYVKKSIYDLGVTQKPTMFGNKVKVYSYERTLCDFIAHKEEMDIEVYVKL
IQSYSSYKERDIHSLYDIAMKMGIENKVREVMEVAYE

Nucleotide

• Length: 594 bp

>AT195471 NZ_CP094733:2295103-2295417 [Staphylococcus epidermidis]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTAGCTGAACTAGGAAGAAAAATCGGTAA
AACTGAAGCTACTGTACAACGTTATGAAAGTGGAAATATCAAAAATCTTAAAAATGATACTATCGAAAGCATAGCTACGG
CATTAAATGTTAACCCTGCATTTTTGATGGGTTGGATAGATGAAAGTGATGAACAACCACAACATCGTGCAGCTCATCTT
GAAGGTGAATTAACAGATGAAGAATGGCAACGTGTGCTAGATTATGCAGATTATATAAGAAGCAAACGCAAATAA

Structures

Toxin

• AlphaFold DB: B6YPW2

Antitoxin

• AlphaFold DB: B6YPW1