Detailed information of TA system TA195466

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 241974..243402
• Replicon: chromosome
• Accession: NZ_CP071144
• Organism: Streptococcus equi subsp. equi strain 470_008

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: B6YPW2
• Locus tag: JZI59_RS01085
• Protein ID: WP_012679622.1
• Coordinates: 241974..242816 (-)
• Length: 281 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: B6YPW1
• Locus tag: JZI59_RS01090
• Protein ID: WP_012679623.1
• Coordinates: 242809..243402 (-)
• Length: 198 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZI59_RS01070 (JZI59_01070)	239205..239951	-	747	WP_050315911.1	hypothetical protein	-
JZI59_RS01075 (JZI59_01075)	239993..240544	-	552	WP_012679620.1	hypothetical protein	-
JZI59_RS01080 (JZI59_01080)	240548..241888	-	1341	Protein_219	DNA topoisomerase	-
JZI59_RS01085 (JZI59_01085)	241974..242816	-	843	WP_012679622.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JZI59_RS01090 (JZI59_01090)	242809..243402	-	594	WP_012679623.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JZI59_RS01095 (JZI59_01095)	243496..245037	-	1542	Protein_222	DUF4366 domain-containing protein	-
JZI59_RS01100 (JZI59_01100)	245096..245383	-	288	WP_012679625.1	conjugal transfer protein	-
JZI59_RS01105 (JZI59_01105)	245396..247684	-	2289	WP_012679626.1	CHAP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	58346..294767	236421

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-268)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (6-51)

Sequences

Toxin

Protein

• Length: 281 a.a.
• Molecular weight: 32683.90 Da
• Isoelectric Point: 5.9694

>T195466 WP_012679622.1 NZ_CP071144:c242816-241974 [Streptococcus equi subsp. equi]
MNKAKLTALCHKVSKEVGLSFNAVMLYYFLESILKKLAGSKYSERFIFKGGFLLSSVVGIDSRSTVDIDFLLRNMQLSEE
NIVQMLNETLKPEEFDDIFYELQNIVPIKEEDQYGGFRANILCKMENIRQIVPLDIATGDVITPHPIDYKYVSSFGEEEI
IIKAYPIETMLAEKIQTIYARGFLNSRSKDYYDLYIIYKLKDKDVNVEILREACRKTFSYRKTEFDIGKIIDLLEKLKIN
EAFLKRWQAYSRKNLYAKDITFEEVLDNGIKMVEKIKNEN

Nucleotide

• Length: 843 bp

>T195466 NZ_CP094731:2033721-2033909 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAGAGAAGAACGAAAAGAATCTTATCTAGGTTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 198 a.a.
• Molecular weight: 23345.76 Da
• Isoelectric Point: 8.6522

>AT195466 WP_012679623.1 NZ_CP071144:c243402-242809 [Streptococcus equi subsp. equi]
MNYENKIEKYLKQSGGIITTAYCKENNIPTIYLSRLLKDGRLSKVRKGIYITKDGDYDEYYFFQHQYKKAIFSYETALYL
LGQTDKIPWSIDVTVYNGYKFNEKPNGFNVHYVKKSIYDLGVTQKPTMFGNKVKVYSYERTLCDFIAHKEEMDIEVYVKL
IQSYSSYKERDIHSLYDIAMKMGIENKVREVMEVAYE

Nucleotide

• Length: 594 bp

>AT195466 NZ_CP094731:2033439-2033696 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATGTATTCAACTTGATATTCACCATTTTATTTTTTACATTTAACATTCTCATAACCTATAA
CGCAAATATTGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGCTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTATATTATATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: B6YPW2

Antitoxin

• AlphaFold DB: B6YPW1